-
Category
Second Step Reagents
-
Long description
Mouse anti-rabbit-HRP. Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
-
Antibody come from
n/a
-
Other description
Provided as solution in phosphate buffered saline pH 7.2 with 0.1% BSA and 0.01% sodium azide.
-
Clone
RG-16
-
Antigen antibody binding interaction
Mouse anti rabbit HRP Antibody
-
Antibody is raised in
Mouse
-
Antibody s reacts with
Rabbit
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Western Blot, ELISA
-
Antibody s suited for
Antibody can be used for the localization of rabbit IgG by ELISA, immunobotting and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions. _x000B__x000B_Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
-
Storage
-20ºC
-
Relevant references
1. Herring, A., et al., Reduction of Amyloid Angiopathy and A Plaque Burden after Enriched Housing in TgCRND8 Mice : Involvement of Multiple Pathways Am. J. Pathol. 169, 544-552, (2006) Abstract_x000B_ 2. Henderson, B., et al., Identification of the Exported Proteins of the Oral Opportunistic Pathogen Actinobacillus actinomycetemcomitans by Using Alkaline Phosphatase Fusions Infect. Immun. 69, 2748-2752, (2001) Abstract_x000B_ 3. Reichard, U., et al., Sedolisins, a New Class of Secreted Proteases from Aspergillus fumigatus with Endoprotease or Tripeptidyl-Peptidase Activity at Acidic pHs Appl. Environ. Microbiol. 72, 1739-1748, (2006) Abstract_x000B_ 4. Palijan, A., et al., Function Of Histone Deacetylase 6 As A Cofactor Of Nuclear Receptor Coregulator LCoR. J. Thorac. Cardiovasc. Surg. 284, 30264-74, (2009)_x000B_ 5. Palijan, A., et al., Ligand-dependent Corepressor LCoR Is An Attenuator Of Progesterone-regulated Gene Expression. J. Thorac. Cardiovasc. Surg. 284, 30275-87, (2009)_x000B_ 6. Chang, J.C., et al., Receptor Guanylyl Cyclases In Inka Cells Targeted By Eclosion Hormone. Proc. Natl. Acad. Sci. U. S. A. 106, 13371-6, (2009) Abstract_x000B_ 7. Herring, A., et al., Environmental Enrichment Enhances Cellular Plasticity In Transgenic Mice With Alzheimer-like Pathology. Experimental Neurology 216, 184-92, (2009) Abstract
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
-
Conjugation
HRP Conjugated
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Oryctolagus cuniculus
-
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
