CD30, 105-120 kD, Clone: mCD30.1, Armenian Hamster Mouse Monoclonal antibody-Mouse; flow/IF
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Catalog numberOBT1506
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PricePlease ask
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Size0.5 mg
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Alternative name 1CD30, 105-120 kD, Clone: mCD30.1, Armenian Hamster Mab anti-Mouse; flow/IF
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Alternative name 2CD30, 105-120 kD, Clone: mCD30.1, Armenian Hamster Mab antibody-Mouse; flow/IF
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Alternative name 3CD30, 105-120 kD, Clone: mCD30.1, Armenian Hamster Mab antibody-Mus musculus; flow/IF
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Alternative name 4anti-CD30 105-120 kD Clone: mCD30.1 Armenian flow/
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Alternative name 5CD30 105-120 kD Clone: mCD30.1 Armenian flow/
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolFAHD2A, COPZ1, TNFRSF8, FYB1, MIR105-2, MIR105-1
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Short nameCD30, 105-120 kD, Clone: mCD30 1, Armenian Mouse Monoclonal antibody-Mouse; /IF
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TechniqueMouse monoclonal, Antibody, Mouse, Flow, IF, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostHamster, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesMouse, Hamsters, Mouses
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Alternative nameCD30, 105-120 kD, clonality: mCD30.1, Armenian Hamster Mouse monoclonal (antibody to-)-Mouse; flow/IF
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene namefumarylacetoacetate hydrolase domain containing 2A
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-08-19
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCOPI coat complex subunit zeta 1
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Synonyms gene
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Synonyms gene name
- coatomer protein complex, subunit zeta
- coatomer protein complex, subunit zeta 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-28
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Entrez gene record
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RefSeq identity
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Classification
- COPI coat complex
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTNF receptor superfamily member 8
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor receptor superfamily, member 8
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-01-10
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Entrez gene record
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Pubmed identfication
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Classification
- CD molecules
- Tumor necrosis factor receptor superfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameFYN binding protein 1
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Synonyms gene
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Synonyms gene name
- FYN-binding protein (FYB-120/130)
- FYN binding protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemicroRNA 105-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR105 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 105-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR105 family
MeSH Data
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Name
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ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data