DNA Quantification Assay Kit (Fluorometric)
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Catalog number
K539-2000
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Price
Please ask
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Size
2000 assays
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Description
Sensitive, specific assay. Protein, RNA and ssDNA do not react with the probe
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Detection Method
Fluorometric (Ex/Em = 492/528 nm)
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Species Reactivity
All
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Applications
Quantification of DNA for rtPCR, qPCR, Next Generation Sequencing (NGS)
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Sample Type
Purified DNA samples
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Features Benefits
Simple, one-step, results in 30 min or less,Sensitive, specific,Does not detect protein, RNA or ssDNA
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
DNA quantification is essential for a variety of applications, such as real-time PCR (rtPCR), qPCR, Next Generation Sequencing (NGS), etc. The measurement of DNA using analytical instruments (i.e. “nanodrop”) is often limited by the presence/contamination of other macromolecules like protein and RNAs found in biological samples. However, the development of highly specific probes that bind to DNA allows the measurement of this macromolecule using a fluorimeter. The biggest advantages of this approach is protein, RNA and single-stranded DNA (ssDNA) are not detected. BioVision’s DNA Quantification Assay Kit provides a quick, specific, and easy method for the measurement of DNA concentrations in a wide variety of samples. In this assay, DNA specifically reacts with a probe producing a stable fluorescence signal (Ex/Em = 492/528 nm). The kit is simple to perform, specific, sensitive and high-throughput adaptable with a wide detection range (0.4-150 ng DNA) and a limit of detection of 4 pg/µl dsDNA in samples.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
DNA Quantification Assay Kit (Fluorometric)
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Technique
Assay, dna, assays
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Host
Assay
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Alternative name
Desoxyribonucleic acid Quantification test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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