IgG, IgA, IgM, H&L (FITC)
-
Catalog number
MBS626627
-
Price
Please ask
-
Size
2 mg
-
-
Products_type
Antibody
-
Products_short_name
[IgG, IgA, IgM, H&L]
-
Products_name_syn
[Anti -IgG, IgA, IgM, H&L (FITC)]
-
Clonality
Polyclonal
-
Reactivity
Human
-
Specificity
Recognizes heavy and light chains on human IgG, IgA and IgM based on IEP.
-
Purity
Affinity Purified Purified by immunoaffinity chromatography.
-
Form
Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).
-
Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
-
Tested_application
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
-
Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
-
Conjugation
Anti-FITC Antibody
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
-
Gene target
-
-
Gene symbol
CALD1, FUT1, L3MBTL2
-
Short name
IgG, IgA, IgM, H L (FITC)
-
Technique
FITC, IgA, IgG, IgM, Fluorescein, IgAs, IgGs, IgMs
-
Host
Goat
-
Isotype
IgG, Heavy & Light chains, IgA, IgG, IgM
-
Label
FITC
-
Alternative name
Immunoglobulin G, Immunoglobulin A, Immunoglobulin M, heavy + light chain (fluorecein)
-
Alternative technique
fluorescine, immunoglobulin-a, immunoglobulins, immunoglobulin-m
-
Gene info
-
Identity
-
Gene
-
Long gene name
caldesmon 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1992-07-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products