Refer to PubMed
Net weight (g)
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Human, Mouse, Rat
Representative figure legend
Western blot analysis of Human 293T cell lysates showing detection of Histone H2A.X protein using Rabbit Anti-Histone H2A.X Polyclonal Antibody (SPC-1251). Lane 1 and Lane 2: Human 293T, Histone2A.X Antibody (Ser139). Lane 3 and Lane 4: Human 293T, Histone2A.X Antibody (pSer139). Primary Antibody: Rabbit Anti-Histone H2A.X Polyclonal Antibody (SPC-1251) at 1:1000. Rabbit Anti-Histone H2A.X Antibody (pSer139) used in Western blot (WB) on 293T cell lysates (SPC-1251)
Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Antibody's other name
H2A histone family member X Antibody, H2A.FX Antibody, H2A.X Antibody, H2AFX Antibody, H2AX histone Antibody, Histone 2A Antibody, Histone 2AX Antibody
Antibody's recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
To be tested
Primary research fields
Cell Signaling, Epigenetics and Nuclear Signalling, Histones, DNA/RNA, DNA Damage and Repair, DNA Damage Recognition
Contact our support service to receive datasheet or other technical documentation.
Total weight (kg)
H2AX (H2A histone family, member X) is one of several genes coding for histone H2A. H2AX becomes phosphorylated on serine 139 as a reaction on DNA Double-strand breaks (DSB). The kinases of the PI3-family (Ataxia telangiectasia mutated, ATR and DNA-PKcs) are responsible for this phosphorylation, especially ATM. The role of the phosphorylated form potentially allows for space for the recruitment of proteins necessary during repair of DSBs. In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals. H2A.X is phosphorylated at Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation.
Human Histone H2A.X (pSer139)
Certificate of analysis
A 1:1000 dilution of SPC-1251 was sufficient for detection of Histone H2A.X in 10 µg of 293T cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
See included datasheet or contact our support service.
Antibody's full description
Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal
Country of production
Antibody's purified from
Peptide Affinity Purified
See included datasheet or contact our support service
To be tested
Antibody in cell
1. Kuo L.J., and Yang L.X. (2008) In Vivo. 22(3): 305-309. 2. Mukherjee B., et al. (2006) DNA Repair (Amst). 5: 575-90. 3. Solier S., et al. (2009) Mol Cell Biol. 29: 68-82. 4. Lu C., et al. (2006) Mol Cell. 23: 121-32. 5. Lu C., et al. (2008) FEBS Lett. 582: 2703-8.
Detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.
Blue Ice or 4°C