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Rabbit Anti Human Histone H2A pSer139 Polyclonal

Rabbit Anti Human Histone H2A pSer139 Polyclonal is available 18 times from Stressmark antibodies labs

SPC-1251D-ALP | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: Alkaline Phosphatase size: 100 µl | 390.99 USD

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SPC-1251D-APC | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: APC size: 100 µl | 397.69 USD

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SPC-1251D-A390 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: ATTO 390 size: 100 µl | 399.92 USD

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SPC-1251D-A488 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: ATTO 488 size: 100 µl | 398.80 USD

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SPC-1251D-A565 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: ATTO 565 size: 100 µl | 398.80 USD

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SPC-1251D-A594 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: ATTO 594 size: 100 µl | 398.80 USD

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SPC-1251D-A633 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: ATTO 633 size: 100 µl | 398.80 USD

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SPC-1251D-A655 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: ATTO 655 size: 100 µl | 398.80 USD

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SPC-1251D-A680 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: ATTO 680 size: 100 µl | 398.80 USD

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SPC-1251D-A700 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: ATTO 700 size: 100 µl | 398.80 USD

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SPC-1251D-BI | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: Biotin size: 100 µl | 393.22 USD

Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test StressMark antibodies supplies antibodies that are for research of human proteins.
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation Biotinylated
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus
Gene targetHistone H2A.X pSer139
Short name Rabbit Anti- Histone H2A.X (pSer139) Polyclonal:
Technique anti-Human, Polyclonal, Rabbit, anti
Label Biotin
Alternative name production species: rabbit antibody to-H. sapiens Histone H2A.X (pSer139) polyclonal: biotinilated
MeSH Data
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Product images
    #Human#.jpg

SPC-1251D-FITC | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: FITC size: 100 µl | 388.75 USD

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SPC-1251D-HRP | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: HRP size: 100 µl | 384.28 USD

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SPC-1251D-P594 | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: PE/ATTO 594 size: 100 µl | 406.62 USD

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SPC-1251D-PCP | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: PerCP size: 100 µl | 397.69 USD

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SPC-1251D-RPE | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: RPE size: 100 µl | 394.34 USD

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SPC-1251D-STR | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: Streptavidin size: 100 µl | 396.57 USD

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SPC-1251D | Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: Unconjugated size: 100 µl | 345.18 USD

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PubMed number Refer to PubMed
Net weight (g) 0.1
Recommended buffer for storage PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Antibody's reactivity Human, Mouse, Rat
Warning information Non-hazardous
Representative figure legend Western blot analysis of Human 293T cell lysates showing detection of Histone H2A.X protein using Rabbit Anti-Histone H2A.X Polyclonal Antibody (SPC-1251). Lane 1 and Lane 2: Human 293T, Histone2A.X Antibody (Ser139). Lane 3 and Lane 4: Human 293T, Histone2A.X Antibody (pSer139). Primary Antibody: Rabbit Anti-Histone H2A.X Polyclonal Antibody (SPC-1251) at 1:1000. Rabbit Anti-Histone H2A.X Antibody (pSer139) used in Western blot (WB) on 293T cell lysates (SPC-1251)
Raised in Rabbit
NCBI number NP_002096.1
Gene number 3014
Released date 6/Sep/2016
Immunogen Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Antibody's other name H2A histone family member X Antibody, H2A.FX Antibody, H2A.X Antibody, H2AFX Antibody, H2AX histone Antibody, Histone 2A Antibody, Histone 2AX Antibody
Protein number P16104
Antibody's recommended dilutions for use WB (1:1000); optimal dilutions for assays should be determined by the user.
Tested applications To be tested
Primary research fields Cell Signaling, Epigenetics and Nuclear Signalling, Histones, DNA/RNA, DNA Damage and Repair, DNA Damage Recognition
Antibody's datasheet Contact our support service to receive datasheet or other technical documentation.
Total weight (kg) 1.4
Stock availabilit In Stock
Scientific context H2AX (H2A histone family, member X) is one of several genes coding for histone H2A. H2AX becomes phosphorylated on serine 139 as a reaction on DNA Double-strand breaks (DSB). The kinases of the PI3-family (Ataxia telangiectasia mutated, ATR and DNA-PKcs) are responsible for this phosphorylation, especially ATM. The role of the phosphorylated form potentially allows for space for the recruitment of proteins necessary during repair of DSBs. In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals. H2A.X is phosphorylated at Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation.
Antibody's target Human Histone H2A.X (pSer139)
Certificate of analysis A 1:1000 dilution of SPC-1251 was sufficient for detection of Histone H2A.X in 10 µg of 293T cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Tissue specificity See included datasheet or contact our support service.
Antibody's full description Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal
Country of production Canada
Antibody's purified from Peptide Affinity Purified
Antibody's concentration See included datasheet or contact our support service
Tested reactivity To be tested
Antibody in cell Nucleus, Chromosome
Antibody's category Polyclonal Antibodies
Bibliography 1. Kuo L.J., and Yang L.X. (2008) In Vivo. 22(3): 305-309. 2. Mukherjee B., et al. (2006) DNA Repair (Amst). 5: 575-90. 3. Solier S., et al. (2009) Mol Cell Biol. 29: 68-82. 4. Lu C., et al. (2006) Mol Cell. 23: 121-32. 5. Lu C., et al. (2008) FEBS Lett. 582: 2703-8.
Verified applications WB, ICC/IF
Antibody's specificity Detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.
Storage recommendations -20°C
Shipping recommendations Blue Ice or 4°C
Host Rabbit
Species Human
Alternative technique polyclonals
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Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: Biotin
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+32-(0)1-658-90-45 [email protected]
  • Histone
  • H2A.X
  • pSer139
  • Alkaline
  • Phosphatase
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