Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal: Biotin

• Catalog number
  SPC-1251D-BI
• Price
  Please ask
• Size
  100 µl

• Immunogen
  Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
• Antibody s target
  Human Histone H2A.X (pSer139)
• Antibody s full description
  Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal
• Primary research fields
  Cell Signaling, Epigenetics and Nuclear Signalling, Histones, DNA/RNA, DNA Damage and Repair, DNA Damage Recognition
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  H2A histone family member X Antibody, H2A.FX Antibody, H2A.X Antibody, H2AFX Antibody, H2AX histone Antibody, Histone 2A Antibody, Histone 2AX Antibody
• Verified applications
  WB, ICC/IF
• Raised in
  Rabbit
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s recommended dilutions for use
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Peptide Affinity Purified
• Recommended buffer for storage
  PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
• Antibody s concentration
  See included datasheet or contact our support service
• Antibody s specificity
  Detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  A 1:1000 dilution of SPC-1251 was sufficient for detection of Histone H2A.X in 10 µg of 293T cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Nucleus, Chromosome
• Tissue specificity
  See included datasheet or contact our support service.
• Scientific context
  H2AX (H2A histone family, member X) is one of several genes coding for histone H2A. H2AX becomes phosphorylated on serine 139 as a reaction on DNA Double-strand breaks (DSB). The kinases of the PI3-family (Ataxia telangiectasia mutated, ATR and DNA-PKcs) are responsible for this phosphorylation, especially ATM. The role of the phosphorylated form potentially allows for space for the recruitment of proteins necessary during repair of DSBs. In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals. H2A.X is phosphorylated at Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation.
• Bibliography
  1. Kuo L.J., and Yang L.X. (2008) In Vivo. 22(3): 305-309. 2. Mukherjee B., et al. (2006) DNA Repair (Amst). 5: 575-90. 3. Solier S., et al. (2009) Mol Cell Biol. 29: 68-82. 4. Lu C., et al. (2006) Mol Cell. 23: 121-32. 5. Lu C., et al. (2008) FEBS Lett. 582: 2703-8.
• Released date
  6/Sep/2016
• NCBI number
  NP_002096.1
• Gene number
  3014
• Protein number
  P16104
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
• Representative figure legend
  Western blot analysis of Human 293T cell lysates showing detection of Histone H2A.X protein using Rabbit Anti-Histone H2A.X Polyclonal Antibody (SPC-1251). Lane 1 and Lane 2: Human 293T, Histone2A.X Antibody (Ser139). Lane 3 and Lane 4: Human 293T, Histone2A.X Antibody (pSer139). Primary Antibody: Rabbit Anti-Histone H2A.X Polyclonal Antibody (SPC-1251) at 1:1000. Rabbit Anti-Histone H2A.X Antibody (pSer139) used in Western blot (WB) on 293T cell lysates (SPC-1251)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Biotinylated
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  Histone   H2A   pSer139  
• Gene symbol
  H2AC20, H2AC8, H2AC7, H2AC18, H2AC17, H2AC11, H2AC19, H2AC15, H2AC4, H2AC14
• Short name
  Rabbit Anti- Histone H2A X (pSer139) Polyclonal:
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG
• Label
  Biotin
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman Histone H2A.X (pSer139) polyclonal: biotinilated
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:4738
• Gene
  H2AC20
• Long gene name
  H2A clustered histone 20
• Synonyms gene
  • H2A
  • H2AFQ
  • HIST2H2AC
• Synonyms gene name
  • H2A histone family, member Q
  • histone 2, H2ac
  • histone cluster 2, H2ac
  • histone cluster 2 H2A family member c
• Synonyms
  • H2A/q
• GenBank acession
  • AY131973
• Locus
  1q21.2
• Discovery year
  1998-08-27
• Entrez gene record
  8338
• Pubmed identfication
  • 1469070
  • 9439656
  • 12408966
• RefSeq identity
  • NM_003517
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000035825

Gene info

• Identity
  HGNC:4724
• Gene
  H2AC8
• Long gene name
  H2A clustered histone 8
• Synonyms gene
  • H2AFA
  • HIST1H2AE
• Synonyms gene name
  • H2A histone family, member A
  • histone 1, H2ae
  • histone cluster 1, H2ae
  • histone cluster 1 H2A family member e
• Synonyms
  • H2A/a
  • H2A.1
• GenBank acession
  • M60752
• Locus
  6p22.2
• Discovery year
  1997-08-29
• Entrez gene record
  3012
• Pubmed identfication
  • 9119399
  • 1916825
  • 12408966
• RefSeq identity
  • NM_021052
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000014440

Gene info

• Identity
  HGNC:4729
• Gene
  H2AC7
• Long gene name
  H2A clustered histone 7
• Synonyms gene
  • H2AFG
  • HIST1H2AD
• Synonyms gene name
  • H2A histone family, member G
  • histone 1, H2ad
  • histone cluster 1, H2ad
  • histone cluster 1 H2A family member d
• Synonyms
  • H2A/g
  • H2A.3
• GenBank acession
  • Z80776
• Locus
  6p22.2
• Discovery year
  1997-08-29
• Entrez gene record
  3013
• Pubmed identfication
  • 9119399
  • 12408966
• RefSeq identity
  • NM_021065
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000014437

Gene info

• Identity
  HGNC:4736
• Gene
  H2AC18
• Long gene name
  H2A clustered histone 18
• Synonyms gene
  • H2AFO
  • HIST2H2AA
  • HIST2H2AA3
• Synonyms gene name
  • H2A histone family, member O
  • histone 2, H2aa
  • histone 2, H2aa3
  • histone cluster 2, H2aa3
  • histone cluster 2 H2A family member a3
• Synonyms
  • H2A.2
  • H2A/q
• GenBank acession
  • K01889
• Locus
  1q21.2
• Discovery year
  1998-09-07
• Entrez gene record
  8337
• Pubmed identfication
  • 6326092
  • 8179821
  • 15527963
• RefSeq identity
  • NM_003516
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000188678

Gene info

• Identity
  HGNC:4735
• Gene
  H2AC17
• Long gene name
  H2A clustered histone 17
• Synonyms gene
  • H2AFN
  • HIST1H2AM
• Synonyms gene name
  • H2A histone family, member N
  • histone 1, H2am
  • histone cluster 1, H2am
  • histone cluster 1 H2A family member m
• Synonyms
  • H2A/n
  • H2A.1
• GenBank acession
  • X57138
• Locus
  6p22.1
• Discovery year
  1998-08-27
• Entrez gene record
  8336
• Pubmed identfication
  • 1768865
  • 9439656
  • 12408966
• RefSeq identity
  • NM_003514
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000014494

Gene info

• Identity
  HGNC:4737
• Gene
  H2AC11
• Long gene name
  H2A clustered histone 11
• Synonyms gene
  • H2AFP
  • HIST1H2AG
• Synonyms gene name
  • H2A histone family, member P
  • histone 1, H2ag
  • histone cluster 1, H2ag
  • histone cluster 1 H2A family member g
• Synonyms
  • pH2A/f
  • H2A/p
  • H2A.1b
• GenBank acession
  • L19778
• Locus
  6p22.1
• Discovery year
  1998-10-05
• Entrez gene record
  8969
• Pubmed identfication
  • 8179821
  • 12408966
• RefSeq identity
  • NM_021064
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000014469

Gene info

• Identity
  HGNC:29668
• Gene
  H2AC19
• Long gene name
  H2A clustered histone 19
• Synonyms gene
  • HIST2H2AA4
• Synonyms gene name
  • histone 2, H2aa4
  • histone cluster 2, H2aa4
  • histone cluster 2 H2A family member a4
• Synonyms
  • H2A/r
• GenBank acession
  • L19779
• Locus
  1q21.2
• Discovery year
  2006-06-12
• Entrez gene record
  723790
• Pubmed identfication
  • 15527963
• RefSeq identity
  • NM_001040874
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000012097

Gene info

• Identity
  HGNC:4726
• Gene
  H2AC15
• Long gene name
  H2A clustered histone 15
• Synonyms gene
  • H2AFD
  • HIST1H2AK
• Synonyms gene name
  • H2A histone family, member D
  • histone 1, H2ak
  • histone cluster 1, H2ak
  • histone cluster 1 H2A family member k
• Synonyms
  • H2A/d
• GenBank acession
  • Z83739
• Locus
  6p22.1
• Discovery year
  1998-08-27
• Entrez gene record
  8330
• Pubmed identfication
  • 9439656
  • 12408966
• RefSeq identity
  • NM_003510
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000016382

Gene info

• Identity
  HGNC:4734
• Gene
  H2AC4
• Long gene name
  H2A clustered histone 4
• Synonyms gene
  • H2AFM
  • HIST1H2AB
• Synonyms gene name
  • H2A histone family, member M
  • histone 1, H2ab
  • histone cluster 1, H2ab
  • histone cluster 1 H2A family member b
• Synonyms
  • H2A/m
• GenBank acession
  • X00089
• Locus
  6p22.2
• Discovery year
  1998-08-27
• Entrez gene record
  8335
• Pubmed identfication
  • 9439656
  • 9119399
  • 12408966
• RefSeq identity
  • NM_003513
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000014420

Gene info

• Identity
  HGNC:4727
• Gene
  H2AC14
• Long gene name
  H2A clustered histone 14
• Synonyms gene
  • H2AFE
  • HIST1H2AJ
• Synonyms gene name
  • H2A histone family, member E
  • histone 1, H2aj
  • histone cluster 1, H2aj
  • histone cluster 1 H2A family member j
• Synonyms
  • H2A/E
• GenBank acession
  • Z83736
• Locus
  6p22.1
• Discovery year
  2003-02-11
• Entrez gene record
  8331
• Pubmed identfication
  • 9439656
  • 12408966
• RefSeq identity
  • NM_021066
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000014486

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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