This antibody has been succesfully tested for use in following methods: Western blot
Target of this antibody: SDF-1a
Alternative names of antibody target: SDF-1a, SDF 1a, SDF 1alpha, SDF 1 alpha, SDF -1, SDF, Stromal cell-derived factor 1;DF-1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; PBSF;SDF-1-alpha
Recommended usage: Western blot analysis (0.5-1 µg/ml). Based on researcher’s feedback, it can also be used in immunofluorescence and neutralization (2-10 µg/ml). However, the optimal conditions should be determined individually.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human, mouse, rat
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: E. coli-expressed human SDF-1α
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: SDF-1α (stromal cell-derived factor-α), also known as pre-B-cell growth-stimulating factor (PBSF), is a 70-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.
Form of antibody: Liquid
Concentration of antibody: 0.5 mg/ml
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps