Human CellExp™ CXADR /CAR, human recombinant
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Catalog number7441-50
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PricePlease ask
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Size50 ug
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SynonymsCXADR, CAR, CAR4/6, HCAR
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Alternative_namesCXADR, CAR, CAR4/6, HCAR
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DescriptionA homophilic and as a heterophilic cell adhesion molecule
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activityMeasured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 50000 cells/well are added to CXADR coated plates (4 ug/mL and 100 uL/well), approximately 20% - 50% will adhere specifically after 60 minutes at 37℃.
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ResultsMeasured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 50000 cells/well are added to CXADR coated plates (4 ug/mL and 100 uL/well), approximately 20% - 50% will adhere specifically after 60 minutes at 37℃.
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Molecular WeightThis protein is fused with a C-terminal 6×His tag, has a calculated MW of 24.9 kDa expressed. The predicted N-terminus is Leu 20. Protein migrates as 35 kDa in reduced SDS-PAGE due to glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationCoxsackie virus and adenovirus receptor (CXADR) also known as CAR, is a type I transmembrane glycoprotein for group B coxsackie viruses and subgroup C adenoviruses, and belongs to the CTX family of the Ig superfamily. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. Human CXADR protein contains a signal sequence, a extracellular domain (ECD) with a V type (D1) and a C2 type (D2) Iglike domain, a transmembrane segment and a intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions, and is necessary and sufficient for adenovirus binding. Variants of CXADR are attached to the cell membrane by a GPI- anchor.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCXADR, CXADRP1, NR1I3, SPG7
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Short nameCellExp™ CXADR /CAR, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ coxsackie virus and adenovirus receptor /CAR, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetcoxsackie virus and adenovirus receptor, CAR and CAR4/6 and HCAR, CXADR and IDBG-854 and ENSG00000154639 and 1525, connexin binding, nuclei, Gm1123 and IDBG-192613 and ENSMUSG00000044860 and 382097, CXADR and IDBG-630474 and ENSBTAG00000004869 and 281733
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Gene info
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Identity
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Gene
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Long gene nameCXADR Ig-like cell adhesion molecule
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Synonyms gene name
- coxsackie virus and adenovirus receptor
- CXADR, Ig-like cell adhesion molecule
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-03-24
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Entrez gene record
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Pubmed identfication
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Classification
- IgCAM CXADR-related subfamily
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCXADR pseudogene 1
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Synonyms gene name
- coxsackie virus and adenovirus receptor pseudogene 1
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Synonyms
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Locus
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Discovery year2008-01-31
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear receptor subfamily 1 group I member 3
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Synonyms gene name
- nuclear receptor subfamily 1, group I, member 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-09-23
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Pubmed identfication
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Classification
- Nuclear receptor subfamily 1 group I
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSPG7 matrix AAA peptidase subunit, paraplegin
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Synonyms gene
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Synonyms gene name
- cell matrix adhesion regulator
- spastic paraplegia 7 (pure and complicated autosomal recessive)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-06-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- AAA ATPases
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
