Recombinant Human Platelet-Derived Growth Factor AA/PDGF-AA Protein(N-6His) 50µg

• Catalog number
  PKSH032905-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  Platelet-derived growth factor subunit A;PDGF subunit A;PDGF-1;Platelet-derived growth factor A chain;Platelet-derived growth factor alpha polypeptide; PDGFA;PDGF1
• Activity
  Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.The ED50 for this effect is less than 300 ng/ml.
• Sequence
  Ser87-Thr211
• Fusion tag
  N-6His
• Accession
  P04085
• Expressed Host
  E.coli
• Shipping
  The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
• Purity
  ＞90% as determined by reducing SDS-PAGE.
• Endotoxin
  ＜1.0 EU per µg as determined by LAL test.
• Stability and Storage
  Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
• Mol Mass
  15.9 kDa
• AP Mol Mass
  16 kDa
• Formulation
  Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
• Reconstitution
  Please refer to it for detailed information.
• Background
  Platelet-derived growth factor subunit A (PDGFA), belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein, stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. It plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis, normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing; Signaling is modulated by the formation of heterodimers with PDGFB.
• Product URL
• PDF URL
• Description
  Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Platelet-Derived   Growth   Factor   AA/PDGF-AA   Protein   N-6His   50µg  
• Gene symbol
  PDGFA, TEAD1
• Short name
  Recombinant Platelet-Derived Growth Factor AA/PDGF-AA Protein(N-6His) 50µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Platelet-Derived Growth Factor AA/PDGF-AA Protein(N-6His) 50µg
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8799
• Gene
  PDGFA
• Long gene name
  platelet derived growth factor subunit A
• Synonyms gene name
  • platelet-derived growth factor alpha polypeptide
• Synonyms
  • PDGF1
  • PDGF-A
• Synonyms name
  • PDGF A-chain
  • platelet-derived growth factor alpha chain
• Locus
  7p22.3
• Discovery year
  1986-01-01
• Entrez gene record
  5154
• Pubmed identfication
  • 1505216
  • 2536956
• VEGA ID
  OTTHUMG00000151412

Gene info

• Identity
  HGNC:11714
• Gene
  TEAD1
• Long gene name
  TEA domain transcription factor 1
• Synonyms gene
  • TCF13
  • AA
• Synonyms gene name
  • atrophia areata, peripapillary chorioretinal degeneration
  • TEA domain family member 1 (SV40 transcriptional enhancer factor)
• Synonyms
  • TEF-1
• Synonyms name
  • transcriptional enhancer factor 1
• GenBank acession
  • X84839
• Locus
  11p15.3
• Discovery year
  1994-09-22
• Entrez gene record
  7003
• Pubmed identfication
  • 1851669
  • 9889009
  • 15016762
• RefSeq identity
  • NM_021961
• Classification
  • TEA domain transcription factors
• VEGA ID
  OTTHUMG00000165878

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products