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Category
Negative Control
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Long description
This ready to use Negative Control reagent contains a combination of fluorescein and phycoerythrin conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human intracellular or cell surface antigens, same low range of nonspecific binding to human leukocytes as other COMBI-IC-Reagents. Like all other COMBI-IC-reagents, this reagent should be used in combination with our FIX&PERM Cell Permeabilization Kit (Cat.No. GAS-002) These isotype control IgG1 are suitable as negative controls to be used in combination with COMBI-IC reagents for the: - Enumeration of Myeloid Cells - Analysis of Myeloid Differentiation Stage - Enumeration of B-cells and Precursors - Enumeration of T-cells and Precursors - Analysis of Leukemia Cells - Analysis of Immunodeficiency States Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation.
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Antibody come from
n/a
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Other description
PBS pH 7.2, 1% BSA, 0.05% NaN3
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Clone
VI-AP and VI-AP
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Antigen antibody binding interaction
Mouse COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE)
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Antibody is raised in
Mouse
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
VI-AP reacts with calf intestine alkaline phosphatase and does not show cross-reactivity with human proteins.
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Research interest
CD Marker
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Application
Flow cytometry
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Antibody s suited for
Permeabilization and Staining Procedure - In combination with our Permeabilization Kit FIX&PERM (Cat. No. GAS-002) intracellular isotype controls can be easily stained in cell suspensions. - For each sample to be analyzed add 50 µl of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube - Add 100 µl of Reagent A (Fixation Medium, stored and used at room temperature) - Incubate for 15 minutes at room temperature - Add 5 ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g - Remove supernatant and add to cell pellet 100 µl Reagent B (Permeabilization Medium) and 20 µl of COMBI-IC Negative Control antibody conjugate. - Vortex at low speed for 1-2 seconds - Incubate for 15 minutes at room temperature - Wash cells with phosphate buffered saline as described above - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 % formaldehyde and store them at 2-8°C in the dark. Analyze fixed cells within 24 hours.
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Storage
Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8°C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.
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Relevant references
J. L. Clarke, W. Watkins, J Biol Chem 271, 10317 (1996). R. N. Knibbs et al., J Cell Biol 133, 911 (1996). B. Kniep et al., J Biochem (Tokyo) 119, 456 (1996). A. J. Wagers, L. M. Stoolman, R. Kannagi, R. Craig, G. S. Kansas, J Immunol 159, 1917 (1997). M. Noguchi, N. Sato, H. Sugimori, K. Mori, K. Oshimi, Leuk Res 25, 847 (2001). W. M. Watkins, J. L. Clarke, Adv Exp Med Biol 491, 231 (2001).
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Protein number
see ncbi
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Warnings
For professional users only. This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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Properties
This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
