Anti-TNF alpha (Polyclonal), ALEXA Fluor 594

  • Catalog number
    GENTObs-2081R-A594
  • Price
    Please ask
  • Size
    100 microliters
  • Type
    Conjugated Primary Antibody
  • Conjugated with
    ALEXA FLUOR® 594
  • Host organism
    Rabbit (Oryctolagus cuniculus)
  • Target Protein Peptide
    TNF alpha
  • Specificity
    This antibody reacts specifically with TNF alpha
  • Modification
    No modification has been applied to this antibody
  • Modification site
    None
  • Clonality
    Polyclonal Antibody
  • Clone
    Polyclonal Antibodies
  • Concentration
    1ug per 1ul
  • Subcellular locations
    Extracellular
  • Antigen Source
    KLH conjugated synthetic peptide derived from mouse TNF-alpha
  • Gene ID
    21926
  • Swiss Prot
    P06804
  • Applications
    IF(IHC-P)
  • Applications with corresponding dilutions
    IF(IHC-P)(1:50-200)
  • Cross reactive species
    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
  • Cross Reactive Species details
    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
  • Background information
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • Purification method
    Purified by Protein A.
  • Storage
    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
  • Excitation emission
    590nm/617nm
  • Synonyms
    DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; Tnf
  • Also known as
    TNF alpha Polyclonal Antibody
  • Other name
    Anti-TNF alpha Polyclonal
  • Advisory
    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
  • Properties
    For facs or microscopy Alexa 1 conjugate.
  • Conjugation
    Alexa Fluor, ALEXA FLUOR® 594
  • Conjugated
    Alexa conjugate 1
  • Description
    The Anti-TNF alpha is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • Gene
    Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
  • Gene target
    TNF   alpha  
  • Gene symbol
    TNF, TNFRSF1A, TNFRSF1B, LTBR
  • Short name
    Anti-TNF alpha
  • Technique
    Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Isotype
    Immunoglobulin G (IgG)
  • Label
    ALEXA FLUOR 594
  • Alternative name
    TNF alpha Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
  • Alternative technique
    polyclonals, antibodies
  • Alternative to gene target
    tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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