Stem Cell Factor, Recombinant, Mouse (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

  • Catalog number
    MBS650726
  • Price
    Please ask
  • Size
    5x0.01 mg
  • Products_type
    Active Protein
  • Products_short_name
    [Stem Cell Factor]
  • Other_names
    [stem cell factor]
  • Purity
    Highly PurifiedHighly purified (98% by SDS-PAGE and HPLC analyses).
  • Form
    Supplied as a lyophilized powder from 10mM sodium phosphate, pH 6.5. Reconstitute with sterile dH2O to a concentration of 0.1-1.0mg/ml with carrier protein (0.1% HSA or BSA).
  • Storage_stability
    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O and carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
  • Description
    For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
  • Latin name
    Mus musculus
  • Source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    Stem   Factor   Stem   Factor   CD117   c-kit   Steel   Factor   Mast   Growth   Factor   MGF  
  • Gene symbol
    STAT5A, KIT, KITLG
  • Short name
    Stem Factor, Recombinant, Mouse (Stem Factor, CD117 c-kit, Steel Factor, Mast Growth Factor, MGF)
  • Technique
    Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    E Coli
  • Species
    Mouse, Mouses
  • Alternative name
    progenitor cellular Factor, Rec., Mouse (progenitor cellular Factor, CD117 c-reagent, Steel Factor, Mast cellular Growth Factor, MGF)
  • Alternative technique
    rec, murine, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell, stem
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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