CHK2 (Thr68) Antibody, ALEXA FLUOR 594

  • Catalog number
    bs-3721R-A594
  • Price
    Please ask
  • Size
    100ul
  • Long name
    CHK2 (Thr68) Polyclonal Antibody, ALEXA FLUOR 594 Conjugated
  • Also known as
    Anti-CHK2 Thr68 PAb ALEXA FLUOR 594
  • Category
    Conjugated Primary Antibodies
  • Conjugated with
    ALEXA FLUOR® 594
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    CHK2 Thr68
  • Specificity
    This is a highly specific antibody against CHK2 Thr68.
  • Modification
    Phosphorylation
  • Modification Site
    Thr68
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Concentration
    1ug per 1ul
  • Immunogen range
    50-90/543
  • Subcellular location
    Nucleus
  • Source
    KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of Thr68
  • Gene ID Number
    11200
  • Swiss Prot
    O96017
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Cross reactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Background of the antigen
    Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.
  • Purification
    Purified by Protein A.
  • Storage conditions
    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
  • Excitation emission
    590nm/617nm
  • Synonyms
    CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Checkpoint kinase 2; CHEK2
  • Properties
    For facs or microscopy Alexa 1 conjugate. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Conjugation
    Alexa Fluor, ALEXA FLUOR® 594
  • Conjugated
    Alexa conjugate 1
  • French translation
    anticorps
  • Gene target
    CHK2   Thr68  
  • Gene symbol
    CHEK2
  • Short name
    Anti-CHK2 Thr68
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Immunoglobulin G (IgG)
  • Label
    ALEXA FLUOR 594
  • Alternative name
    CHK2 (Thr68) Polyclonal Antibody, ALEXA FLUOR 594
  • Alternative technique
    antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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