Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens). Other species not known. ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
FACS: 1-2ug/million cells,IF: 1-2ug/ml,
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1X Phosphate Buffered Saline (PBS), pH 7.4
This CD8a antibodyis to be used only for research purposes and not for diagnostics..
Gene ID #
Purified by protein A Affinity Chromatography
CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha-beta) or as a homodimer (CD8 alpha-alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8 alpha antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
Human CD8 recombinant fragment corresponding to aa 200-250 was used as the immunogen for this antibody.
Store the CD8a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Antibodies to CD8a
Alternative to gene target
CD8a molecule, CD8 and Leu2 and MAL and p32, CD8A and IDBG-59987 and ENSG00000153563 and 925, protein homodimerization activity, Extracellular, Cd8a and IDBG-155582 and ENSMUSG00000053977 and 12525, CD8A and IDBG-634911 and ENSBTAG00000021141 and 281060