Other name
DNA damage-inducible transcript 3 protein,DDIT-3,C/EBP zeta,C/EBP-homologous protein,CHOP,C/EBP-homologous protein 10,CHOP-10,CCAAT/enhancer-binding protein homologous protein,Growth arrest and DNA damage-inducible protein GADD153,DDIT3,CHOP,CHOP10,GADD153
Immunogen
Recombinant human DNA damage-inducible transcript 3 protein (1-169AA)
Verified reactivity
Human
Verified applications
ELISA,IHC. Recommended dilution:IHC:1:20-1:200
Raised in
Rabbit
Clonality
Polyclonal
Protein number
P35638
Gene number
Please refer to GenBank
NCBI number
Please refer to NCBI
Purity
Protein G purified, >95%
Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
Use before
1 year
Shipping requirements
Blue ice
Estimated production time
3-7 business days
Supplementary information
Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.
Notes
For research use only. Not for diagnostic procedures.
Properties
If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation
anticorps
Gene targetDDIT3
Short name
DDIT3 Polyclonal Antibody
Technique
Polyclonal, Antibody
Label
Unconjugated
Alternative name
Desoxyribonucleic acid-damage-inducible transcript 3 polyclonal (Antibody to)
Alternative technique
polyclonals
Alternative to gene target
DNA-damage-inducible transcript 3, CEBPZ and CHOP and CHOP-10 and CHOP10 and GADD153, DDIT3 and IDBG-42805 and ENSG00000175197 and 1649, sequence-specific DNA binding, nuclei, Ddit3 and IDBG-194880 and ENSMUSG00000025408 and 13198, DDIT3 and IDBG-635159 and ENSBTAG00000031544 and 777788
