Rabbit Anti-Human Histone H3 Polyclonal: Biotin
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Catalog numberSPC-1252D-BI
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PricePlease ask
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Size100 µl
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ImmunogenA synthesized peptide derived from human Histone H3
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Antibody s targetHuman Histone H3
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Antibody s full descriptionRabbit Anti-Human Histone H3 Polyclonal
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Primary research fieldsCell Signaling, Epigenetics and Nuclear Signalling, Histones
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameH3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
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Verified applicationsWB, IHC, ICC/IF
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useWB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects endogenous levels of Histone H3
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-1252 was sufficient for detection of Histone H3 in 10 µg of COLO205 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cellNucleus, Chromosome
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextHistone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
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Bibliography1. Wu R., et al. (1981) Cell. 27:321. 2. Strahl B., et al. (2000) Nature. 403:41. 3. Bauer U., et al. (2002) EMBO Rep. 3:39. 4. Frank D., et al. (2003) Gene. 312:135.
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Released date6/Sep/2016
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NCBI numberNP_003520.1
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Gene number8350
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Protein numberP68431/Q71DI3/P84243
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Human COLO205 cell lysates showing detection of ~17kDa Histone H3 protein using Rabbit Anti-Histone H3 Polyclonal Antibody (SPC-1252). Lane 1: Human COLO205 cell lysate treated with the immunizing peptide. Lane 2: Human COLO205 cell lysate. Primary Antibody: Rabbit Anti-Histone H3 Polyclonal Antibody (SPC-1252) at 1:1000. Predicted/Observed Size: ~17kDa. Rabbit Anti-Histone H3 Antibody used in Western blot (WB) on COLO205 cell lysates (SPC-1252)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolH3-4, H3C15, H3-7
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Short nameRabbit Anti- Histone H3 Polyclonal:
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman Histone H3 polyclonal: biotinilated
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameH3.4 histone
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Synonyms gene
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Synonyms gene name
- H3 histone family, member T
- histone 3, H3
- histone cluster 3, H3
- histone cluster 3 H3
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH3 clustered histone 15
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Synonyms gene
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Synonyms gene name
- histone 2, H3a
- histone cluster 2, H3a
- histone cluster 2 H3 family member a
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-19
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Entrez gene record
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Pubmed identfication
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Classification
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH3.7 histone (putative)
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Synonyms gene
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Synonyms gene name
- histone 2, H3, pseudogene 2
- histone cluster 2, H3, pseudogene 2
- histone cluster 2 H3 pseudogene 2
- H3.2 histone (putative)
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-06-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H3 histones
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
