CD4, T-Cell, Helper/Inducer, Clone: RM4-5, Rat Mouse Monoclonal antibody-Mouse, FITC

  • Catalog number
    ACL016F-3
  • Price
    Please ask
  • Size
    0.3mg
  • Alternative name 1
    CD4, T-Cell, Helper/Inducer, Clone: RM4-5, Rat Mab anti-Mouse, FITC
  • Alternative name 2
    CD4, T-Cell, Helper/Inducer, Clone: RM4-5, Rat Mab antibody-Mouse, FITC
  • Alternative name 3
    CD4, T-Cell, Helper/Inducer, Clone: RM4-5, Rattus norvegicus Mab antibody-Mus musculus, FITC
  • Alternative name 4
    anti-CD4 T-Cell Hel r/Inducer Clone: RM4-5
  • Alternative name 5
    CD4 T-Cell Hel r/Inducer Clone: RM4-5
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
  • Additional description
    CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. For cells, cell lines and tissues in culture till half confluency.
  • Conjugation
    Anti-FITC Antibody
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    CD4, MIR1302-5, PIRC22, PIRC23, PIRC20, PIRC21, HTR6, IGKV3OR2-5, IGHV2OR16-5, SNORD116-5
  • Short name
    CD4, T- , Helper/Inducer, Clone: RM4-5, Mouse Monoclonal antibody-Mouse, FITC
  • Technique
    Mouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    Rat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Label
    FITC
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    CD4 molecule, T-cellular, Helper/Inducer, clonality: RM4-5, Rat Mouse monoclonal (antibody to-)-Mouse, fluorecein
  • Alternative technique
    antibodies, murine, fluorescine
  • Alternative to gene target
    CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
  • Tissue
    cell
Gene info
  • Identity
  • Gene
    CD4
  • Long gene name
    CD4 molecule
  • Synonyms gene name
    • CD4 antigen (p55)
    • T-cell surface glycoprotein CD4
  • GenBank acession
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
    920
  • RefSeq identity
  • Classification
    • CD molecules
    • C2-set domain containing
    • V-set domain containing
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 22
  • Locus
    5
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 23
  • Locus
    5
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 20
  • Locus
    5
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 21
  • Locus
    5
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    5-hydroxytryptamine receptor 6
  • Synonyms gene name
    • 5-hydroxytryptamine (serotonin) receptor 6
    • 5-hydroxytryptamine (serotonin) receptor 6, G protein-coupled
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1997-12-11
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • 5-hydroxytryptamine receptors, G protein-coupled
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 3/OR2-5 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 3/OR2-5
    • immunoglobulin kappa variable 3/OR2-5 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • Classification
    • Immunoglobulin kappa (IGK) orphons
  • VEGA ID
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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