Alternative names of antibody target:
CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen=CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form.
Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the “adapter molecule,” lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.
Clonality of antibody:
This antibody reacts with:
This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-322 amino acids from the C-terminal region of human CD14.
Concentration of antibody:
Formulation of this antibody:
100 µl of antibody in PBS with 0.09% (W/V) sodium azide
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
This antibody has been succesfully tested for use in following methods:
Western blot, IHC, FACS
Western blot: ~1:1000, IHC: ~1:10–1:50, FACS: ~1:10-1:50.
Long gene name mannose-6-phosphate receptor, cation dependent
Synonyms gene name
- mannose-6-phosphate receptor (cation dependent)
Discovery year 1989-06-30
Entrez gene record 4074
Havana BLAST/BLAT OTTHUMG00000168276
Long gene name CD14 molecule
Discovery year 1988-05-31
Entrez gene record 929
- CD molecules
- Scavenger receptors
Havana BLAST/BLAT OTTHUMG00000129507
Long gene name cardiotrophin 1
Discovery year 1995-05-01
Entrez gene record 1489
- Interleukin 6 type cytokine family
Havana BLAST/BLAT OTTHUMG00000132413