Phospho-Cyclin E1 (Thr77) Antibody

  • Catalog number
    A1154-100
  • Price
    Please ask
  • Size
    100 µl
  • Antibody Target
    CCNE1
  • Target Alternative Name
    CCNE; G1/S-specific cyclin-E1
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human, Mouse
  • Immunogen Sequence
    KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin E1
  • Accession
    P24864
  • Gene Id
    898
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide
  • Purification
    Affinity purified
  • Application
    WB, IH, IF/IC
  • Positive Control
    WB: HEK293; IHC: human heart tissue; IFC: HEK293 cells
  • Application And Usages
    WB; 1:500 – 1:2000, IHC; 1:50 – 1:200, IF/IC; 1:50 – 1:100
  • Description
    Cyclin E1 is a regulatory subunit of the CDK2 kinase. It is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
  • Storage Condition
    -20°C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans
  • Handling
    The antibody solution should be gently mixed before use
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Verified
  • Immunofluorescence Verified
    Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
  • Short name
    Phospho-Cyclin E1 (Thr77) Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    phosphorilated-Cyclin E1 (Thr77) (antibody to-)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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