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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr588 of human EphA2 (AA585-591)
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Antibody s target
Human EphA2 (pTyr588)
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Antibody s full description
Rabbit Anti-Human EphA2 (pTyr588) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Eck antibody, Eph receptor A2 antibody, EPHA2 antibody, Ephrin receptor antibody, Ephrin receptor EphA2 antibody, Ephrin type A receptor 2 antibody, Epithelial cell kinase antibody, Myk 2 antibody, Sek 2 antibody, Tyrosine protein kinase receptor ECK antibody, Tyrosine-protein kinase receptor MPK-5 antibody, Tyrosine-protein kinase receptor SEK-2 antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 108.266 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-966 was sufficient for detection of EphA2 (pTyr588) in 10 µg of HepG2 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
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Scientific context
EphA2 is a receptor-tyrosine kinase that is activated upon binding ephrins of the ephrin A family. It regulates cell adhesion, migration, proliferation and differentiation. It also may be involved in apoptosis, induced by UV-radiation.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_004422
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Gene number
1969
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Protein number
P29317
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
RPE
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Short name
Rabbit Anti- EphA2 (pTyr588) Polyclonal: RPE
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
RPE
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman EPH receptor A2 (pTyr588) polyclonal: RPE
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
EPH receptor A2, ARCC2 and CTPA and CTPP1 and CTRCT6 and ECK, EPHA2 and IDBG-91407 and ENSG00000142627 and 1969, transferase activity, Cell surfaces, Epha2 and IDBG-202005 and ENSMUSG00000006445 and 13836, EPHA2 and IDBG-645870 and ENSBTAG00000000815 and 512798
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