Mouse anti VCAM-1

• Catalog number
  MUB1905P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Vascular cell adhesion molecule-1 (VCAM-1) is induced on Human umbilical vein endothelium (HUVE) by recombinant Human tumor necrosis factor - α (rh TNF-α), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigRation at sites of inflammation and immune reaction.
• Antibody come from
  4B9 is a Mouse IgG1 monoclonal antibody geneRated by immunization of Balb/c mice with Human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant Human (rh) TNF-α (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  4B9
• Antigen antibody binding interaction
  Mouse anti VCAM-1 Antibody
• Antibody is raised in
  Mouse (Balb/c)
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).
• Research interest
  Cancer,Immunology
• Application
  Flow Cytometry,Immunohistochemistry (frozen),Immunoprecipitation
• Antibody s suited for
  4B9 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Carlos. T., Schwartz. B., Kovach. N., Yee. E., Rosa. M., Osborn. L., Chi-Rosso. G., Newman. B., Lobb. R., et al. (1990). Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured Human endothelial cells Blood. 76(5) 965-70. Ockenhouse, C.F., Tegoshi, T., Maeno, Y., Benjamin, C., Ho, M., Kan, K.E., Thway, Y., Win, K., Aikawa, M. and Lobb, R.R. (1992). Human vascular endothelial cell adhesion receptors for Plasmodium falciparum-infected erythrocytes: roles for endothelial leukocyte adhesion molecule 1 and vascular cell adhesion molecule 1. J Exp Med. 176, 1183-9. Bacchi, C.E., Marsh, C.L., Perkins, J.D., Carithers, R.L. Jr, McVicar, J.P., Hudkins, K.L., Benjamin, C.D., Harlan, J.M., Lobb, R. and Alpers, C.E. (1993). Expression of vascular cell adhesion molecule (VCAM-1) in liver and pancreas allograft rejection. Am J Pathol. 142, 579-91. Pinola, M., Saksela, E., Tiisala, S. and Renkonen, R. (1994). Human NK cells expressing alpha 4 beta 1/beta 7 adhere to VCAM-1 without preactivation. Scand J Immunol. 39, 131-6. Chuluyan, H.E., Osborn, L., Lobb, R. and Issekutz AC. (1995). Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte trans-endothelial migRation. J Immunol. 155, 3135-4.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  VCAM-1  
• Gene symbol
  VCAM1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
• Short name
  Mouse anti VCAM-1
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to VCAM-1
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:12663
• Gene
  VCAM1
• Long gene name
  vascular cell adhesion molecule 1
• Synonyms
  • CD106
• GenBank acession
  • M60335
• Locus
  1p21.2
• Discovery year
  1991-07-10
• Entrez gene record
  7412
• RefSeq identity
  • NM_001078
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • Receptor ligands
  • C2-set domain containing
  • I-set domain containing
• VEGA ID
  OTTHUMG00000010982

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

Gene info

• Identity
  HGNC:31545
• Gene
  MIR16-1
• Long gene name
  microRNA 16-1
• Synonyms gene
  • MIRN16-1
• Synonyms
  • hsa-mir-16-1
• Locus
  13q14.2
• Discovery year
  2004-04-23
• Entrez gene record
  406950
• RefSeq identity
  • NR_029486
• Classification
  • MicroRNA MIR15/16 family

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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