JMJD2A Antibody

• Catalog number
  6108-100
• Price
  Please ask
• Size
  100 μl

• Antibody Target
  JMJD2A
• Target Alternative Name
  Jumonji Domain Containing 2A; KDM4A; Lysine-Specific Demethylase 4A
• Antibody Type
  Polyclonal
• Species Reactivities
  Human JMJD2A. Expected to cross react with JMJD2C (>90% similarity), JMJD2B (>85% similarity) due to high homology to these JMJD2 paralogs considering the antigen used. Observed detection of purified recombinant human JMJD2D at 50 ng sμggesting only ~10% cross reactivity with recombinant JMJD2D, however bands were detected at 60 kDa from cell samples.
• Immunogen Sequence
  Human recombinant JMJD2A aa 1-350
• Accession
  O75164
• Gene Id
  9682
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µl of antibody in TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide.
• Purification
  Antigen affinity purified
• Application
  Western blot
• Positive Control
  Western blot: DLD1 cell lysate
• Application And Usages
  Western blot: 1:200 dilution. Not recommended for FACS and IHC based on probable cross reactivity with other JMJD containing proteins.
• Description
  Jumonji Domain containing 2A (JMJD2A) is a lysine specific demethylase with emerging roles in histone modification or epigenetic remodeling. This JMJD2A polyclonal antibody was raised against an N-terminal recombinant fragment of JMJD2A. This fragment includes amino acids 1-350 including the JMJN and JMJC domains but not the two LAP/PHD zinc finger or Tudor domains of the 1,064 amino acid protein. JMJD2A is detected at 135 kDa by western blotting using a DLD1 cell lysate as a positive control. Also, the expression of JMJD2A varies by tissue, and northern blotting sμggests skeletal muscle as a negative control and lung as a positive control tissue.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  JMJD2A  
• Gene symbol
  KDM4A
• Short name
  JMJD2A Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  JMJD2A (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:22978
• Gene
  KDM4A
• Long gene name
  lysine demethylase 4A
• Synonyms gene
  • JMJD2
  • JMJD2A
• Synonyms gene name
  • jumonji domain containing 2
  • jumonji domain containing 2A
  • lysine (K)-specific demethylase 4A
• Synonyms
  • KIAA0677
  • JHDM3A
  • TDRD14A
• Synonyms name
  • jumonji C domain-containing histone demethylase 3A
  • tudor domain containing 14A
• GenBank acession
  • AB014577
• Locus
  1p34.2-p34.1
• Discovery year
  2003-08-08
• Entrez gene record
  9682
• Pubmed identfication
  • 9734811
  • 15138608
• RefSeq identity
  • NM_014663
• Classification
  • Tudor domain containing
  • Lysine demethylases
• VEGA ID
  OTTHUMG00000007560

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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