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Category
Other Reagents
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Long description
BRAK is a novel member of the CXC chemokine family. The function of BRAK in cells remains largely unknown. It was identified by translating expressed sequence tags (ESTs) derived from breast and kidney carcinoma libraries and was found to be expressed ubiquitously in RNA from normal tissue extracts but absent from a variety of established in vitro tumor cell lines.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from HeLa cells and mouse myeloma cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
Brak-Biotin - Control Lysate
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Antibody is raised in
see techfile
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Antibody s reacts with
Human CXC
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Activity Assay
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Antibody s suited for
Antibody detects cytokeration protein numbers 13 and 10 (molecular weights 53 and 56.6 kDa, respectively, according to Moll's classification) by Western blot. Does not react with other cytokeratins or other proteins. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Ivanyi, D., et al. Modulation of mammary carcinoma cell phenotype and keratin expression patterns by retinoic acid. Cancer Lett, 1993, 73, 191-205._x000B__x000B_2. Ivanyi, D., et al. Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas. Am. J. Vet. Res. 1993, 54, 1095-1102._x000B__x000B_3. Ivanyi, D., et al. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. American Journal of Veterinary Research, 1992, 53, 304-314._x000B__x000B_4. Ivanyi, D., et al. Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis. Cancer Research, 1990, 50, 5143-5152._x000B__x000B_5. Ivanyi, D., et al. New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva. J of Pathol, 1989, 159, 7-12._x000B__x000B_6. Moll, R., et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
