Anti-Rabbit IgG, Fc Specific-Biotin Polyclonal antibody

• Catalog number
  84401
• Price
  Please ask
• Size
  1mg

• Clonality
  Polyclonal antibody
• Long name
  Polyclonal antibody against Rabbit IgG, Fc Specific-Biotin
• Aplication
  You will receive the antybody in PBS(7,4 pH), for future delutions PBS or mediun similar to the one used for the assay are suitable.
• Antigen
  Rabbit IgG, Fc Specific-Biotin
• Storage
  Could be stored for at least 12 month in frozen condition at -70C.It is recomendable to prepare aliquotes in order to avoid repetable freezing and thawing as they may denaturate the peptide chains/bonds in the antibodies and reduce/change the activity of the antibody. Prior to use briefly centrifuge the vial to collect its content on the bottom.
• Product description
  Anti-Rabbit IgG, Fc Specific-Biotin Polyclonal antibody can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by QED Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by QED Biosciences. Rabbit antibodies are very stable and can be stored for several days at room temperature. QED Biosciences adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  Polyclonals and antibodies
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  Specific  
• Short name
  Rabbit IgG, Fc Specific-Biotin pAb
• Technique
  Polyclonal, Antibody, Rabbit, anti-, anti, IgG, FC, antibody to, antibodies, antibodies against human proteins, antibodies for, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Label
  Biotin
• Alternative name
  Antibody tohost: rabbit Immunoglobulin G, fragment c Specific-biotinilated polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies, rabbit-anti, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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