Recombinant Human CAMK1D / CKLiK Protein (GST tag)

  • Catalog number
    PKSH030390-20µg
  • Price
    Please ask
  • Size
    20µg
  • Synonym
    CaM-K1;CaMKID;CKLiK
  • Activity
    The specific activity was determined to be 70 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate (see Activity Assay Protocol)
  • Sequence
    Met 1-Lys 385
  • Fusion tag
    N-GST
  • Accession
    NP_705718.1
  • Expressed Host
    Baculovirus-Insect Cells
  • Shipping
    Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
  • Purity
    >80 % as determined by SDS-PAGE
  • Endotoxin
    <1.0 EU per µg as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    69 kDa
  • AP Mol Mass
    60 kDa
  • Formulation
    Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM Reduced Glutathione, pH 8.0
  • Background
    Calcium/calmodulin-dependent protein kinase or CaM kinases are serine/threonine-specific protein kinases that are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. In neurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction of long-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse. Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of the protein kinase superfamily and CaMK subfamily. It contains one protein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    CAMK1D   CKLiK   Protein   GST   tag  
  • Gene symbol
    CAMK1D, MGST3
  • Short name
    Recombinant CAMK1D / CKLiK Protein (GST tag)
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Label
    tag
  • Species
    Human, Humans
  • Alternative name
    Rec. H. sapiens calcium/calmodulin-dependent protein phosphorylation catalyst ID / CKLiK Protein (GST detection labelled)
  • Alternative technique
    rec
  • Alternative to gene target
    calcium/calmodulin-dependent protein kinase ID, CAMK1D and IDBG-54250 and ENSG00000183049 and 57118, transferase activity, nuclei, Camk1d and IDBG-134225 and ENSMUSG00000039145 and 227541, CAMK1D and IDBG-636735 and ENSBTAG00000008650 and 526873
Gene info
  • Identity
  • Gene
  • Long gene name
    calcium/calmodulin dependent protein kinase ID
  • Synonyms gene name
    • calcium/calmodulin-dependent protein kinase ID
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2003-11-05
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • MicroRNA protein coding host genes
    • Calmodulin dependent protein kinases
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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