LIF, murine recombinant
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Catalog number4924-1000
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PricePlease ask
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Size1 mg
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SynonymsLeukemia Inhibitory Factor
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Alternative_namesLeukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, INN=Emfilermin
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DescriptionA cytokine that affects cell growth by inhibiting differentiation
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Purity≥95%
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Molecular Weight20.0 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from sterile solution containing 20 mM phosphate buffer, pH 7.4 and 0.02% Tween-20
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile H₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffers.
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Background InformationLeukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Leukemia Inhibitory Factor (LIF) Murine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.
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Amino acid sequenceMSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQR KKLGCQLLGTYKQVISVVVQAF
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolLIF-AS1, LIF-AS2, LIF
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Short nameLIF, murine recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative nameleukemia inhibitory factor, murine Rec.
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Alternative techniquerec
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Alternative to gene targetleukemia inhibitory factor, CDF and DIA and HILDA and MLPLI, LIF and IDBG-3968 and ENSG00000128342 and 3976, growth factor activity, Extracellular, Lif and IDBG-142317 and ENSMUSG00000034394 and 16878, LIF and IDBG-636335 and ENSBTAG00000007424 and 280840
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Gene info
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Identity
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Gene
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Long gene nameLIF antisense RNA 1
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Synonyms
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Locus
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Discovery year2017-04-28
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Pubmed identfication
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene nameLIF antisense RNA 2
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Locus
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Discovery year2020-10-07
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene nameLIF interleukin 6 family cytokine
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Synonyms gene name
- leukemia inhibitory factor
- LIF, interleukin 6 family cytokine
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Synonyms
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Synonyms name
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Locus
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Discovery year1989-06-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- Interleukin 6 type cytokine family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Methods or procedures used to obtain samples of URINE.
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Tree numbers
- E01.370.225.998.762
- E05.200.998.762
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
