Pure-IP™ Western Blot Detection test kit

• Catalog number
  PRB-5002
• Price
  Please ask
• Size
  20 blots

• We send this reagant on
  Room temperature
• The temperatureerature for storage is
  Store the HRP conjugate at -20°C. Store the remaining components at 4°C.
• Technical file
  more kit.pdf
• SDS or safty data sheet
  details (PRB-5002) GHS.pdf
• Type of reagent
  Cell Signaling and Protein Biology
• Related techniques
  Cell Signaling and Protein Biology, Protein Analysis Tools, IP Western Blot Detection
• Use of the reagent
  Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection test kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.
• Conjugation and form
  host specific
• Species compatible to
  human cells and cell lines and other cells
• Use of this test kit or product
  Molecular biology
• Antigen
  the sequence of the protein
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,19
• Molecular weight
  kiloDalton
• This reagent is purified
  0.98
• Test
  Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
• Description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. The ultrapure Pure-IP™ Blot test kit supplied from Cellular biology labs is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.

• Gene target
  IP™   test   kit  
• Short name
  Pure-IP™ Blot test kit
• Technique
  Western Blot, detection, Blot, Pure, IP, western Blots, Immunoprecipitation, pure products
• Alternative name
  Pure-IP™ Western Blot quantification test reagent
• Alternative technique
  tests, western, purified, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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