-
Synonyms
IgG Fc
-
Alternative_names
IgG Fc
-
Description
Member of immunoglobulin G secreted by B cells
-
Recombinant
Yes
-
Source
HEK 293 cells
-
Purity by SDS PAGE
≥ 92% by SDS- PAGE
-
Assay
SDS-PAGE
-
Endotoxin Level
< 1.0 EU per 1 ug of protein as determined by LAL method
-
Molecular Weight
This protein carries no "tag". The protein has a calculated MW of 26 kDa. The protein migrates as 33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Lyophilized
-
Physical form description
Lyophilized from sterile PBS, pH 7.4. 5% trehalose, 5% mannitol, 0.01% Tween-80 are added as protectants before lyophilization.
-
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 250 µg/ml. Do not vortex. Divide into small aliquots and store at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
-
Background Information
Immunoglobulin G3 (IgG3) is a member of immunoglobulin G developed and secreted by effective B cells. In the wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a highly conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc/IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
-
Amino acid sequence
The target protein is expressed with the sequence (aa Glu 97 - Lys 329) of Mouse IgG3 Fc region.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
