Recombinant Human SRPK1 Protein (His & GST tag)
-
Catalog number
PKSH030864-20µg
-
Price
Please ask
-
Size
20µg
-
-
Synonym
RP3-422H11.1;SFRSK1
-
Activity
NA
-
Sequence
Glu 2-Ser 655
-
Fusion tag
N-His & GST
-
Accession
AAH38292.1
-
Expressed Host
Baculovirus-Insect Cells
-
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
-
Purity
＞80 % as determined by SDS-PAGE
-
Endotoxin
＜1.0 EU per µg as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
102 kDa
-
AP Mol Mass
120 kDa
-
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, 2mM GSH, 10% gly, pH 7.4
-
Background
Serine / threonine-protein kinase SRPK1, also known as SFRS protein kinase 1, Serine/arginine-rich protein-specific kinase 1, SR-protein-specific kinase 1 and SRPK1, is a cytoplasm and nucleus protein which belongs to the protein kinase superfamily and CMGC Ser/Thr protein kinase family. Isoform 2 of SRPK1 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 of SRPK1 is only seen in the testis, at lower levels than isoform 2. SRPK1 hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. SRPK1 plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. SRPK1 locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. SRPK1 appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.
-
-
Conjugation
histidine
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
SRPK1, MGST3
-
Short name
Recombinant SRPK1 Protein (His & GST tag)
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Label
His
-
Species
Human, Humans
-
Alternative name
Rec. H. sapiens SRSF protein kinase 1 Protein (histidine & GST detection labelled)
-
Alternative technique
rec
-
Alternative to gene target
SRSF protein kinase 1, SFRSK1, SRPK1 and IDBG-84414 and ENSG00000096063 and 6732, transferase activity, nuclei, Srpk1 and IDBG-158718 and ENSMUSG00000004865 and 20815, SRPK1 and IDBG-629997 and ENSBTAG00000016838 and 512631
-
Gene info
-
Identity
-
Gene
-
Long gene name
SRSF protein kinase 1
-
Synonyms gene name
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1998-03-02
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
