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Target antigen
alphaSmooth Muscle Actin
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Clonality
Monoclonal antibody
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Clone
1A4
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Raised in
mice
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Type of the antibody
IgG2a monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human, rat
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Analyses
WB,IHC-P,IHC-F
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Immunogen
N-terminal synthetic decapeptide of alpha-smooth muscle actin.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the alphaSmooth Muscle Actin Antibody ( 1A4)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The alphaSmooth Muscle Actin Antibody ( 1A4) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Ueyama et al.(1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.
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Related articles
1. Kumar, M. S.; Hendrix, J. A.; Johnson, A. D.; Owens, G. K. : Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins. Circ. Res. 92: 840-847, 2003. 2. Ueyama, H.; Bruns, G.; Kanda, N. : Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome 10. Jpn. J. Hum. Genet. 35: 145-150, 1990.
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Gene Name
ACTA2
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Protein Name
Actin, aortic smooth muscle
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Gene Full Name
actin, alpha 2, smooth muscle, aorta
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Synonyms
AAT6|ACTSA|MYMY5|ACTSA|alpha-cardiac actin|cell growth-inhibiting gene 46 protein
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Uniprot ID
Human(P62736), Mouse(P62737), Rat(P62738)
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Entrez GeneID
Human(59), Mouse(11475), Rat(81633)
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
