U-118 MG Cell Line
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Catalog numberEP-CL-0458
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationU-118 MG
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age50 years
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MorphologyMixed
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundNOTE: The two glioblastoma cell lines, U-118 MGand U-138 MG, reportedly from different individuals have identical VNTR and similar STR patterns.U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.Mycoplasma contamination was eliminated in 1987 by treatment with BM-Cycline over a six week culture period.
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Biosafety1
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MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in nude mice inoculated subcutaneously with 10^7 cells. Tumors developed within 21 days at 100% frequency(5/5).
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolIGKV1OR2-118, IGKV1OR15-118, MRPL48, HLA-U, UTS2, CENPU
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Short nameU-118 MG Line
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SpeciesHomo sapiens, Human
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Alternative nameU-118 MG cellular Line
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TissueBrain
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-118 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1/OR2-118 pseudogene
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Synonyms
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Locus
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Discovery year2009-11-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR15-118 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR15-118
- immunoglobulin kappa variable 1/OR15-118 pseudogene
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Synonyms
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
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Identity
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Gene
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Long gene namemitochondrial ribosomal protein L48
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-10-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Large subunit mitochondrial ribosomal proteins
- Mitochondrial ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, U (pseudogene)
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Synonyms gene
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Synonyms gene name
- HLA-21 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-01-16
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Entrez gene record
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameurotensin 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-03-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecentromere protein U
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Synonyms gene
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Synonyms gene name
- MLF1 interacting protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
