Rat CD49e antibody (biotin)

Applications

FC, IHC
Product Type

Primary Antibodies
Product Subtype

Conjugated Monoclonal Antibodies
Research Area

Immunology
Immunogen

CD49e antibody (biotin) was raised in rat using C57BL/6 x A/J F1 murine mast cell line MC/9 as the immunogen.
Specificity

Murine CD49e
Cross Reactivity

To be determined by end-user
Clone

5H10.27
Concentration

0.5 mg/ml
Form Buffer

Supplied as a liquid in PBS, with NaN3 added as preservative.
Storage

Store at 4 deg C.
Shipping Info

Blue Ice
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name

Rattus norvegicus
French translation

anticorps

Identity

HGNC:6141
Gene

ITGA5
Long gene name

integrin subunit alpha 5
Synonyms gene
- FNRA
Synonyms gene name
- integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Synonyms
- CD49e
Synonyms name
- fibronectin receptor, alpha polypeptide
Locus

12q13.13
Discovery year

1988-07-19
Entrez gene record

3678
Pubmed identfication
- 2454952
Classification
- Integrin alpha subunits
- CD molecules
VEGA ID

OTTHUMG00000169841