Sheep anti-human Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG

  • Catalog number
    MBS512152
  • Price
    Please ask
  • Size
    100ug
  • Alternative name1
    Anti-Sheep anti-human Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG
  • Alternative name2
    Ovine anti-human Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG
  • Alternative name3
    Ovinae anti-Homo sapiens Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG
  • Alternative name4
    Thrombin
  • Alternative name5
    Thrombin, human
  • Other names
    thrombin; N/A
  • Gene name
    N/A
  • Gene name synonims
    N/A
  • Other gene names
    N/A
  • Category
    Antibodies
  • Clonality
    Polyclonal
  • Immunoglobulin isotype
    N/A
  • Clone
    Polyclonal antibody
  • Host organism
    Sheep
  • Species reactivity
    N/A
  • Specificity and cross reactivity
    Prior to conjugation, this antibody was specific for thrombin (including prothrombin) as demonstrated by immunoelectrophoresis and ELISA.
  • Purification method
    Affinity purified, FITC conjugated IgG. Vial containing mL of affinity-purified IgG conjugated to fluorescein isothiocyanate (FITC). Total protein is 0.1 mg.
  • Form Appearance
    APIgG-FITC conjugate as a clear yellow liquid.
  • Concentration
    APIgG-FITC concentration is mg/mL, determined by absorbance using an extinction coefficient (E1%280) of 14.
  • Storage and shipping
    Store at 2 degree C to 8 degree C and protect from light.
  • Tested for
    N/A
  • Description
    productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
  • Advisory
    In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
  • Test
    A high affinity purification column was use to purify anti- Thrombin (FIIa), FITC-Conjugated -Purified IgG by MBS Polyclonals by chromatographic size exclusion. MBS Polyclonals supplies antibodies that are for research of human proteins.
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Gene target
  • Short name
    anti- Thrombin (FIIa), FITC-Conjugated -Purified IgG
  • Technique
    anti-Human, affinity, purified, anti-, anti, FITC, IgG, antibody to, antibodies, antibodies against human proteins, antibody Conjugates, Fluorescein, IgGs, purifications
  • Host
    Sheep
  • Isotype
    IgG, IgG
  • Label
    FITC
  • Species
    Human, Humans, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
  • Alternative name
    ovine Antibody tohuman Thrombin (FIIa), fluorecein-coupled protein-enriched Immunoglobulin G
  • Alternative technique
    pure, antibodies, fluorescine, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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