ETO polyclonal antibody

• Catalog number
  6834-50
• Price
  Please ask
• Size
  50 μl

• Antibody Target
  ETO
• Target Alternative Name
  RUNX1T1, AML1T1, CBFA2T1, CDR, MTG8, ZMYND2
• Antibody Type
  Polyclonal
• Species Reactivities
  Human.
• Immunogen Sequence
  Polyclonal antibody against human ETO using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.
• Accession
  Q06455
• Gene Id
  862
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS with 0.05% (W/V) sodium azide.
• Purification
  Whole Anti-Sera
• Application
  ELISA, ChIP.
• Positive Control
  ELISA: Peptides, ChIP: SKNO-1 cells.
• Application And Usages
  ChIP: 4 µl/ChIP, ELISA: 1:100.
• Description
  ETO is a transcriptional regulator which belongs to the myeloid translocation gene family. ETO exerts its function by interaction with transcription factors bound to promoters and binding to histone deacetylases. It recruits a range of corepressors to facilitate transcriptional repression. The t (8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. This translocation produces a chimeric gene made up of the 5’-region of AML1 and the 3’-region of the ETO gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Verified
• ChIP Verified
  Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  ETO  
• Gene symbol
  RUNX1T1
• Short name
  ETO polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Rabbit IgG
• Alternative name
  ETO polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:1535
• Gene
  RUNX1T1
• Long gene name
  RUNX1 partner transcriptional co-repressor 1
• Synonyms gene
  • AML1T1
  • CBFA2T1
• Synonyms gene name
  • core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related
  • runt related transcription factor 1; translocated to, 1 (cyclin D related)
  • RUNX1 translocation partner 1
• Synonyms
  • CDR
  • ETO
  • MTG8
  • ZMYND2
• GenBank acession
  • D43638
• Locus
  8q21.3
• Discovery year
  1993-12-16
• Entrez gene record
  862
• Pubmed identfication
  • 1391946
  • 9790752
• RefSeq identity
  • NM_004349
  • NM_175635
• Classification
  • Zinc fingers MYND-type
• VEGA ID
  OTTHUMG00000164066

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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