HLA B27 Real-TM Real Time PCR kit

Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Gene target

HLA B27 Real-TM Real Time kit
Gene symbol

MT-TM, TSPAN16, ACP3, P4HTM, HLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DQA2, HLA-DPB1
Short name

HLA B27 Real-TM Real Time PCR kit
Technique

PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

Human leukocyte antigen B27 Real-TM Quantitative real-time PCR test kit reagent
Alternative technique

kits, dna-amplification
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:28858
Gene

P4HTM
Long gene name

prolyl 4-hydroxylase, transmembrane
Synonyms gene name
- prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Synonyms
- P4H-TM
- PHD4
- PH4
- HIFPH4
- FLJ20262
- EGLN4
- PH-4
Synonyms name
- Prolyl hydroxlase domain-containing 4
- hypoxia inducible factor prolyl 4 hydroxylase
Locus

3p21.31
Discovery year

2008-12-08
Entrez gene record

54681
Pubmed identfication
- 12163023
- 17726031
RefSeq identity
- NM_177938
VEGA ID

OTTHUMG00000074057

Identity

HGNC:4939
Gene

HLA-DPA2
Long gene name

major histocompatibility complex, class II, DP alpha 2 (pseudogene)
Synonyms gene
- HLA-DP2A
GenBank acession
- X01634
Locus

6p21.32
Discovery year

1986-01-01
Entrez gene record

646702
Pubmed identfication
- 6597088
Classification
- Histocompatibility complex
VEGA ID

OTTHUMG00000031061
Locus Specific Databases
- IMGT/HLA Database

Name

Blood Grouping and Crossmatching
Concept

Scope note: Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. Crossmatching is performed prior to transfusion.
Tree numbers
- E01.370.225.625.120
- E01.370.225.812.385.120
- E05.200.625.120
- E05.200.812.385.120
- E05.478.594.385.120
Qualifiers

ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data