Caspase-13 Substrate LEED-AFC

  • Catalog number
    1116-200
  • Price
    Please ask
  • Size
    200 assays
  • Alternative_name
    Ac-LEED-AFC, Caspase-13 Substrate, Fluorogenic
  • Description
    Ready-to-use fluorometric substrate for caspase-13 and related caspases that recognize the amino acid sequence LEED. Caspase-13 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEED-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
  • Structure Available
    No
  • Peptide sequence
    Ac-Leu-Glu-Glu-Asp-AFC
  • Salt Form
    No
  • Molecular Formula
    C₃₂H₃₈F₃N₅O₁₃
  • Molecular Weight
    757.66
  • Cell Permeable
    No
  • Purity
    >98% by HPLC
  • Handling
    Protect from light and moisture
  • Tag Line
    A caspase-13 substrate
  • Storage Condition
    -20°C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • MDL Number
    MFCD03452816
  • PubChem CID
    44135106
  • SMILES
    CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C
  • InChi
    InChI=1S/C32H38F3N5O13/c1-14(2)10-21(36-15(3)41)31(52)39-19(6-8-24(42)43)28(49)38-20(7-9-25(44)45)29(50)40-22(13-26(46)47)30(51)37-16-4-5-17-18(32(33,34)35)12-27(48)53-23(17)11-16/h4-5,11-12,14,19-22H,6-10,13H2,1-3H3,(H,36,41)(H,37,51)(H,38,49)(H,39,52)(H,40,50)(H,42,43)(H,44,45)(H,46,47)
  • InChi Key
    ZPNCQMJLTSFJQH-UHFFFAOYSA-N
  • MSDS Available
    Yes
  • Additional description
    Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
MeSH Data
  • Name
  • Concept
    Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
  • Tree numbers
    • E05.599.495
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