TWEAK, human recombinant

• Catalog number
  4360-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  APO3L, DR3LG, TWEAK, TNFSF13
• Alternative_names
  APO3L, DR3LG, TWEAK, TNFSF13
• Description
  An apoptosis inducer
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Biological activity
  The ED₅₀ as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml.
• Molecular Weight
  17.0 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 0.1 M L-Arg.
• Reconstitution Instructions
  Reconstitute in 10 mM Sodium Phosphate (pH 7.5) to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
• Background Information
  Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many other members of the TNF family, is able to induce interleukin-8 synthesis in a number of cell lines. The recombinant human TWEAK is a 17.0 kDa protein, comprising the TNF-like extraCellular domain of TWEAK.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  TWEAK  
• Gene symbol
  TNFSF12
• Short name
  TWEAK, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  TWEAK, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11927
• Gene
  TNFSF12
• Long gene name
  TNF superfamily member 12
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 12
• Synonyms
  • TWEAK
  • DR3LG
  • APO3L
• GenBank acession
  • AF030099
• Locus
  17p13.1
• Discovery year
  1998-12-04
• Entrez gene record
  8742
• Pubmed identfication
  • 9405449
  • 9560343
• RefSeq identity
  • NM_003809
• Classification
  • Tumor necrosis factor superfamily
• VEGA ID
  OTTHUMG00000108148
• Locus Specific Databases
  • LRG_1320

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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