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Category
Other Reagents
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Long description
LYMPHOGRAM¨ is a three-color direct immunofluorescence reagent for use in flow cytometry designed to simultaneously determine in peripheral blood, bone marrow and other body fluids the major lymphocyte subpopulations, including the total number of T lymphocytes (CD3+), B lymphocytes (CD19+) and natural killer cells (CD3-CD56+) as well as helper/inducer (CD3+CD4+) and suppressor/cytotoxic (CD3+CD8+) T lymphocyte subsets._x000B__x000B_Flow Cytometry is a powerful tool for the analytical and quantitative characterization of cells which provides rapid, quantitative and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cells in liquid suspension that have been incubated with fluorescently-labeled antibodies directed against specific cellular proteins. The relative fluorescence intensity of the positive cells indicates the amount of antibody bound to specific binding sites on the cells, and therefore provides a relative measure of antigen expression._x000B_Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer (NK) cells. T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. B lymphocytes (CD19+) are the producers of antibodies, they mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. NK cells (CD3-CD56+) mediate cytotoxicity against certain tumors and virus-infected cells. NK-mediated cytotoxicity does not require class I or class II major histocompatibility complex (MHC) molecules to be present on the target cell._x000B_LYMPHOGRAM¨ recognizes the antigens CD3, CD19, CD56, CD4 and CD8 present in the different lymphocyte subsets, and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc (1-3).
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Antibody come from
n/a
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Other description
No other information available
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Clone
not specified
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Antigen antibody binding interaction
Mouse anti Lymphogram(tm)
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Antibody is raised in
Mouse
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Flow Cytometry
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Antibody s suited for
WARNINGS AND RECOMMENDATIONS_x000B_1.For in vitro diagnostic use. _x000B_2.This product is supplied ready to use. If it is altered by dilution or addition of other components, it will be invalidated for in vitro_x000B_diagnostic use. _x000B_3.The reagent is stable until the expiration date shown on the label if it is properly stored. Do no use after the expiration date shown_x000B_on the label. If the reagents are stored in conditions different from those recommended, such conditions must be validated by the user._x000B_LYMPHOGRAM¨ Ref: CYT-C001_x000B_For In Vitro Diagnostic use_x000B_4.Alteration in the appearance of the reagent, such as the precipitation or discoloration indicates instability or deterioration. In such cases, the reagent should not be used._x000B_5.It contains 0.1% sodium azide (CAS-Nr. 26628-22-8) as a preservative, but even so care should be taken to avoid microbial contamination of reagent or incorrect results may occur._x000B_¥Sodium azide (NaN3) is harmful if swallowed (R22), if swallowed, seek medical advice immediately and show this container or label (S46)._x000B_¥Wear suitable protecting clothing (S36). ¥Contact with acids liberates very toxic gas (R32). ¥Azide compounds should be flushed with large volumes of water during disposal to avoid deposits in metal drains where_x000B_explosive conditions may develop. _x000B_6.All patient specimens and materials with which they come into contact are considered biohazards and should be handled as if_x000B_capable of transmitting infection (6), and disposed according to the legal precautions established for this type of product. Also recommended is handling of the product with appropriate protective gloves and clothing, and its use by personnel sufficiently qualified for the procedures described. Avoid contact of samples with skin and mucous membranes. After contact with skin, wash immediately with plenty of water._x000B_7.Use of the reagent with incubation times or temperatures different from those recommended may cause erroneous results. Any such changes must be validated by the user._x000B__x000B_WARRANTY_x000B_This product is warranted only to conform to the quantity and contents stated on the label. There are no warranties that extend beyond the description on the label of the product. CytognosÕs sole liability is limited to either replacement of the product or refund of the purchase price.
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Storage
4ºC
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Relevant references
1.Orfao A, Gonz‡lez de Buitrago JM La citometr’a de flujo en el laboratorio cl’nico. Sociedad espa–ola de bioqu’mica cl’nica y patolog’a Molecular 1995._x000B_2. Stetler-Stevenson M. Flow cytometry analysis of lymphomas and lymphoproliferative disorders. Semin Hematol 2001 Apr;38(2):111-23._x000B_3.Okuno SH, Tefferi A, Hanson C, Katzmann JA, Li CY, Witzig TE. Spectrum of diseases associated with increased proportions or absolute numbers of peripheral blood natural killer cells. BrJ Haematol. 93: 810-812 (1996)_x000B_4.MenŽndez P, et al. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry (Comm. Clin. Cytometry) 34: 264-271 (1998)_x000B_5.Gratama JW, MenŽndez P, Kraan J, Orfao A. Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erytrocyte lysing reagents. J Immunol. Methods 239: 13-23 (2000)_x000B_6.Protection of Laboratory Workers from occupationally acquired infections. Second edition; approved guideline (2001). Villanova PA: National Committee for Clinical Laboratory Standards; Document M29-A2._x000B_7.Procedures for the collection of diagnostic blood specimens by venipuncture- approved standard; Fifth edition (2003). Wayne PA: National Committee for Clinical Laboratory Standards; Document H3-A5._x000B_8.Clinical applications of flow cytometry: Quality assurance and immunophenotyping of lymphocytes; approved guideline (1998). Wayne PA: National Committee for Clinical Laboratory Standards; Document H42-A._x000B_9.Mandy F, Brando B. Enumeration of absolute cell counts using immunophenotypic techniques. Current Protocols in Cytometry 6.8.1-6.8.26 (2000)_x000B_10.Brando B, et al. Cytofluorometric methods for assessing absolute numbers of cells in blood. For the European Working Party on Clinical Cell Analysis (EWGCCA). Cytometry 42: 327-346 (2000)_x000B_11. Bellido M, Rubiol E, †beda J, Estivill C, L—pez O, Manteiga R, NomdedŽu JF. Rapid and simple immunophenotypic characterization of lymphocytes using a new test. Haematologica 83: 681-685 (1998)_x000B_12. Giustolisis GM, Gruszka-Westwood AM, Morilla RM, Matutes E. Lymphogram: a rapid flow cytometry method for screening patients with lymphocytosis. Haematologica 86: 1223-1224 (2001)_x000B_13.Ruiz-Arguelles A, PŽrez-Romano B. Immunophenotypic analysis of peripheral blood lymphocytes. Current Protocols in Cytometry 6.5.1-6.5.14 (2000)_x000B_14. Braylan RC, Orfao A, Borowitz MJ, Davis BH. Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting. Cytometry 46: 23-7 (2001)_x000B_15.Reichert et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol 60:190-208 (1991) 16. Prince HK et al. Influence of racial background on the distribution of T-cell subsets and Leu-11 positive lymphocytes in healthy_x000B_blood donors. Diagn Immunol. 3: 33-39 (1985) 17.Kotylo PK et al. Reference ranges for lymphocyte subsets in pediatric patients. Am J Clin Pathol 100:111-5 (1993)
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
