Caspase-3 Antibody
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Catalog number3138-100
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PricePlease ask
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Size100 µg
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Antibody TargetCaspase-3
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Target Alternative NameCPP32 ,CASP3, apopain, procaspase3,CPP32B,SCA-1,CPP-32,Apopain,Yama
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide corresponding to the C-terminus of caspase-3 large fragment
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AccessionP42574
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Gene Id836
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AppearanceColorless liquid
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FormLiquid
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Concentration0.2 mg/ml
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Formulation0.5 mg/ml affinity purified rabbit anti-caspase-3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA, 0.03% Proclin
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects proform and the cleaved large fragment of caspase-3.
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DescriptionCaspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-3 is synthesized as an inactive pro-enzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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French translationanticorps
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Gene target
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Gene symbolCASP1P3, CASP3, CASP3P1
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Short nameCaspase-3 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namecaspase-3 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namecaspase 1 pseudogene 3
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Synonyms gene name
- caspase 1, apoptosis-related cysteine peptidase pseudogene 3
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Locus
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Discovery year2012-02-24
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namecaspase 3
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Synonyms gene name
- caspase 3, apoptosis-related cysteine protease
- caspase 3, apoptosis-related cysteine peptidase
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-07-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Small nucleolar RNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 3 pseudogene 1
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Synonyms gene name
- caspase 3, apoptosis-related cysteine peptidase pseudogene 1
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Locus
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Discovery year2011-11-22
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
