Caspase-3 Antibody (Clone C33)
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Catalog number
3004-100
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Price
Please ask
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Size
100 µg
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Target Alternative Name
CPP-32 CPP32B SCA-1 apopain CASP3 Yama CPP32 procaspase3
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Antibody Type
Monoclonal
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Clone
C33
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Species Reactivities
Human, mouse, rat
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Immunogen Sequence
Recombinant human Caspase-3
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Accession
P42574
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Gene Id
836
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
0.2 mg/ml
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Formulation
100 µg (200 µg/ml) in PBS containing 50% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
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Purification
Affinity purified
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Application
Western blot
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Application And Usages
Western blotting (0.5-4 µg/ml). The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. However, the optimal conditions should be determined individually.
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Description
The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
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Storage Condition
-20ºC
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Shipping Condition
Gel Pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
true
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Immunocytochemistry Verified
false
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Immunofluorescence Verified
false
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Immunoprecipitation Verified
false
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FACS Verified
false
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ELISA Verified
false
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ChIP Verified
false
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Dot Blot Verified
false
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Flow Cytometry Verified
false
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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French translation
anticorps
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Gene target
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Short name
Caspase-3 Antibody (Clone C33)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Mouse IgG1
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Alternative name
caspase-3 (antibody to-) (clonality C33)
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Alternative technique
antibodies
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Clone name
C33
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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