Caspase-3 Antibody (Clone C33)

  • Catalog number
    3004-100
  • Price
    Please ask
  • Size
    100 µg
  • Target Alternative Name
    CPP-32 CPP32B SCA-1 apopain CASP3 Yama CPP32 procaspase3
  • Antibody Type
    Monoclonal
  • Clone
    C33
  • Species Reactivities
    Human, mouse, rat
  • Immunogen Sequence
    Recombinant human Caspase-3
  • Accession
    P42574
  • Gene Id
    836
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    0.2 mg/ml
  • Formulation
    100 µg (200 µg/ml) in PBS containing 50% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
  • Purification
    Affinity purified
  • Application
    Western blot
  • Application And Usages
    Western blotting (0.5-4 µg/ml). The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. However, the optimal conditions should be determined individually.
  • Description
    The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
  • Storage Condition
    -20ºC
  • Shipping Condition
    Gel Pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    true
  • Immunocytochemistry Verified
    false
  • Immunofluorescence Verified
    false
  • Immunoprecipitation Verified
    false
  • FACS Verified
    false
  • ELISA Verified
    false
  • ChIP Verified
    false
  • Dot Blot Verified
    false
  • Flow Cytometry Verified
    false
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Additional description
    Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
  • French translation
    anticorps
  • Gene target
    Caspase-3   C33  
  • Short name
    Caspase-3 Antibody (Clone C33)
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Mouse IgG1
  • Alternative name
    caspase-3 (antibody to-) (clonality C33)
  • Alternative technique
    antibodies
  • Clone name
    C33
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee