Synthetic peptide corresponding to the residues of human FKBP51
Antibody's other name
AIG6 Antibody, FK506 binding protein 5 Antibody, FKBP5 Antibody, FKBP54 Antibody, HSP90 binding immunophilin Antibody, p54 Antibody, Pplase Antibody, Ptg10 Antibody, Rotamase Antibody, T cekk FK506 binding protein Antibody
Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking
Human, Mouse, Rat, Rabbit, Dog, Hamster
WB (1:2000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Protein G Purified
Antibody buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
Blue Ice or 4ºC
Antibody certificate of analysis
A 1:2000 dilution was sufficient for detection of FKBP51 in ~50 µg total protein using WB analysis.
Antibody in cell
Cytoplasm , Nucleus
See included datasheet or contact our support service
HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F.(2003) J. Biol. Chem. 278(19): 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600. 3. Wu B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7): 3194-3201. 5. Hubler T.R. et al. (2003) Endocrinology 144(6): 2380- 2387.
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B (SMC-138). Tissue: MK cells. Species: Mouse. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody (SMC-138) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. Courtesy of: the Hospital Henri Mondor, France. Western Blot analysis of Human HeLa cell lysates showing detection of FKBP51 protein using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B (SMC-138). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody (SMC-138) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-FKBP51 Antibody [Hi51B] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse MK cells (SMC-138) Mouse Anti-FKBP51 Antibody [Hi51B] used in Western Blot (WB) on Human HeLa cell lysates (SMC-138)
Country of production
Total weight (kg)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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