Human Recombinant AHA1 Protein
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Catalog numberSPR-309A
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PricePlease ask
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Size50 µg
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Stock availabilityIn Stock
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Scientific contextAha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
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Protein targetAHA1
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Protein reactivityHuman
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Certificate of analysisThis product has been certified >90% pure using SDS PAGE analysis. 2uM SPR-309 generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)
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Protein descriptionHuman Recombinant AHA1 Protein
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Other nameAha1 Protein, SHSA1 Protein, HSPC322 Protein, p38 Protein
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Primary research areaCancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
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CategoryProtein
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Brand namenone
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OriginRecombinant
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NCBI numberNP_036243.1
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Gene number10598
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Protein numberO95433
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Verified applicationsWB, SDS-PAGE, Functional Assay
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Relevant bio activityAHA1 Protein
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Protein expression modelE. coli
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Protein charastericsSee included datasheet.
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Peptide sequenceSHMAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF
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Protein purificationAffinity Purified
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Purity pourcentage>90% High purity
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Recommended buffer for storage20mM HEPES buffer pH7.2, 80mM NaCl, 10% glycerol
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Protein concentrationLot/batch specific. See included datasheet.
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Protein specificity~38 kDa
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Protein tagHis tag
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Supplementary useful informationPlease see included datasheet or contact us
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Protein cell localizationCytoplasm
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Bibliography1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
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Release date31-Jul-2009
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PubMed numberNot added. Please refer to PubMed
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Tested applicationsto be tested
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Tested species reactivityto be tested
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Representative figure legendSDS PAGE of human his-tagged Aha1 protein (SPR-309). SDS-Page of Human AHA1 Protein (SPR-309)
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WarningsNon-hazardous materials
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Protein originCanada
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Total weight kg1.4
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Net weight g0.05
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolAHSA1, AHSA2P
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Short nameRecombinant AHA1 Protein
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameH. sapiens Rec. AHA1 Protein
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameactivator of HSP90 ATPase activity 1
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Synonyms gene
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Synonyms gene name
- chromosome 14 open reading frame 3
- AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-07-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameactivator of HSP90 ATPase homolog 2, pseudogene
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Synonyms gene
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Synonyms gene name
- AHA1, activator of heat shock 90kDa protein ATPase homolog 2 (yeast)
- activator of HSP90 ATPase homolog 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-04-04
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data