BRG1 Antibody

  • Catalog number
    3739-100
  • Price
    Please ask
  • Size
    100 µl
  • Antibody Target
    BRG1
  • Target Alternative Name
    BRG1, BRM/SWI2-Related Gene 1, SMARCA4, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, member 4, SNF2L4, Sucrose Nonfermenting 2-Like 4
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human, mouse
  • Immunogen Sequence
    Synthetic peptide from a portion of human BRG1/SMARCA4 protein
  • Accession
    P51532
  • Gene Id
    6597
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    100 µl purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
  • Purification
    Affinity purified
  • Application
    Western Blot
  • Application And Usages
    Western blotting (1:500 – 1:2500 µg/ml). However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the BRG1/SMARCA4 of human and mouse origins. Reactivity to other species has not been tested.
  • Description
    The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRG1 is a member of the SWI/SNF protein family, which forms part of a large ATP-dependent chromatin remodeling complex. This complex is required for transcriptional activation of genes normally repressed by chromatin. BRG1 is mutated in many cancer cell lines, such as breast, prostate, lung, pancreas and colon. Further, BRG1 has an important role as a tumor suppressor. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    BRG1  
  • Gene symbol
    SMARCA4
  • Short name
    BRG1 Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    BRG1 (antibody to-)
  • Alternative technique
    antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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