This antibody has been succesfully tested for use in following methods: Western blot
Target of this antibody: PIM1
Formulation of this antibody: 100 µg (0.5 mg/ml) affinity purified rabbit anti-PIM1 polyclonal antibody in phosphate (PBS, pH 7.2) containing 30% glycerol, 0.5 % BSA and 0.01% thimerosal.
Alternative names of antibody target: PIM, PIM1, PIM-1, Proviral Integration Site 1
Recommended usage: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~33 kDa of PIM1 isoform from samples of human, mouse and rat origins. Reactivity to other species has not been determined.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human, mouse, rat
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Synthetic peptide surrounding amino acid 296 of rat PIM1
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved throμgh evolution in multicellular organisms. Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis.
Form of antibody: Liquid
Concentration of antibody: 0.5 mg/ml
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps
Short name anti-PIM1 Antibody
Technique Antibody, anti
Alternative name antibody to-pim-1 oncogene (Antibody to)
Alternative technique antibodies
Alternative to gene target pim-1 oncogene, PIM, PIM1 and IDBG-85488 and ENSG00000137193 and 5292, transferase activity, nuclei, Pim1 and IDBG-160725 and ENSMUSG00000024014 and 18712, PIM1 and IDBG-630304 and ENSBTAG00000000396 and 281402