Rat CYP7A1(Cytochrome P450 7A1) ELISA Kit

• Catalog number
  ELK7308
• Price
  Please ask
• Size
  96T

• Alternative Names
  CYP7; CYP7-A1; CYPVII; Cholesterol 7 Alpha Hydroxylase; Cytochrome P450 Family 7 Subfamily A Polypeptide 1
• Assay Type
  Sandwich
• Sensitivity
  0.54ng/mL
• Standard
  100ng/mL
• Detection range
  1.56-100ng/mL
• Assay length
  3h
• Research Area
  Metabolic pathway;
• Test principle
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 7A1 (CYP7A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 7A1 (CYP7A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 7A1 (CYP7A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 7A1 (CYP7A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  CYP7A1   Cytochrome   P450   7A1  
• Gene symbol
  CYP7A1, CYP3A7, CYP24A1, CYP1A1, CYP51A1, CYP2D6
• Short name
  CYP7A1(Cytochrome P450 7A1) ELISA Kit
• Technique
  ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
• Host
  Rat, antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Rat, Rats
• Alternative name
  Rat CYP7A1(Cytochrome P450 7A1) Enzyme-linked immunosorbent assay reagent
• Alternative technique
  elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:2651
• Gene
  CYP7A1
• Long gene name
  cytochrome P450 family 7 subfamily A member 1
• Synonyms gene
  • CYP7
• Synonyms gene name
  • cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1
  • cytochrome P450, family 7, subfamily A, polypeptide 1
• Synonyms name
  • cholesterol 7 alpha-monooxygenase
  • cholesterol 7α-hydroxylase
• GenBank acession
  • M89803
• Locus
  8q12.1
• Discovery year
  1992-04-16
• Entrez gene record
  1581
• Pubmed identfication
  • 1358792
• RefSeq identity
  • NM_000780
• Classification
  • Cytochrome P450 family 7
• VEGA ID
  OTTHUMG00000164301

Gene info

• Identity
  HGNC:2640
• Gene
  CYP3A7
• Long gene name
  cytochrome P450 family 3 subfamily A member 7
• Synonyms gene name
  • cytochrome P450, subfamily IIIA, polypeptide 7
  • cytochrome P450, family 3, subfamily A, polypeptide 7
• Synonyms
  • CP37
  • P450-HFLA
• GenBank acession
  • AF315325
• Locus
  7q22.1
• Discovery year
  1992-09-14
• Entrez gene record
  1551
• Pubmed identfication
  • 2722762
• Classification
  • Cytochrome P450 family 3
• VEGA ID
  OTTHUMG00000156726
• Locus Specific Databases
  • PharmVar Human Cytochrome P450 (CYP) Allele Nomenclature

Gene info

• Identity
  HGNC:2602
• Gene
  CYP24A1
• Long gene name
  cytochrome P450 family 24 subfamily A member 1
• Synonyms gene
  • CYP24
• Synonyms gene name
  • cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
  • cytochrome P450, family 24, subfamily A, polypeptide 1
• Synonyms
  • CP24
  • P450-CC24
• GenBank acession
  • U60669
• Locus
  20q13.2
• Discovery year
  1992-09-09
• Entrez gene record
  1591
• Classification
  • Cytochrome P450 family 24
• VEGA ID
  OTTHUMG00000032773

Gene info

• Identity
  HGNC:2595
• Gene
  CYP1A1
• Long gene name
  cytochrome P450 family 1 subfamily A member 1
• Synonyms gene
  • CYP1
• Synonyms gene name
  • cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1
  • cytochrome P450, family 1, subfamily A, polypeptide 1
• Synonyms
  • P450DX
  • P1-450
  • P450-C
  • CP11
• GenBank acession
  • BC023019
• Locus
  15q24.1
• Discovery year
  1986-01-01
• Entrez gene record
  1543
• Pubmed identfication
  • 15128046
• RefSeq identity
  • NM_000499
• Classification
  • Cytochrome P450 family 1
• VEGA ID
  OTTHUMG00000142812
• Locus Specific Databases
  • PharmVar Human Cytochrome P450 (CYP) Allele Nomenclature
  • LRG_1262

Gene info

• Identity
  HGNC:2649
• Gene
  CYP51A1
• Long gene name
  cytochrome P450 family 51 subfamily A member 1
• Synonyms gene
  • CYP51
• Synonyms gene name
  • cytochrome P450, 51 (lanosterol 14-alpha-demethylase)
  • cytochrome P450, family 51, subfamily A, polypeptide 1
• Synonyms
  • CP51
  • CYPL1
  • P450L1
  • LDM
  • P450-14DM
• GenBank acession
  • U51685
• Locus
  7q21.2
• Discovery year
  1996-10-26
• Entrez gene record
  1595
• Pubmed identfication
  • 8975714
• Classification
  • Cytochrome P450 family 51
• VEGA ID
  OTTHUMG00000131131

Gene info

• Identity
  HGNC:2625
• Gene
  CYP2D6
• Long gene name
  cytochrome P450 family 2 subfamily D member 6
• Synonyms gene
  • CYP2DL1
  • CYP2D7P2
  • CYP2D7BP
  • CYP2D8P2
  • CYP2D7AP
• Synonyms gene name
  • cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing), polypeptide 6
  • cytochrome P450, family 2, subfamily D, polypeptide 7 pseudogene 2
  • cytochrome P450, subfamily II (debrisoquine, sparteine, etc., -metabolising), polypeptide 7 pseudogene 2
  • cytochrome P450, family 2, subfamily D, polypeptide 8 pseudogene 2
  • cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolising), polypeptide 8 pseudogene 2
  • cytochrome P450, family 2, subfamily D, polypeptide 6
• Synonyms
  • CPD6
  • P450-DB1
  • CYP2D
  • P450C2D
• GenBank acession
  • M20403
• Locus
  22q13.2
• Discovery year
  1992-04-07
• Entrez gene record
  1565
• Pubmed identfication
  • 8449513
• Classification
  • Cytochrome P450 family 2
• VEGA ID
  OTTHUMG00000150918
• Locus Specific Databases
  • PharmVar Human Cytochrome P450 (CYP) Allele Nomenclature
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database
  • LRG_303

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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