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Products_type
Antibody
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Products_short_name
[PCNA (Proliferating Cell Nuclear Antigen)]
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Products_name_syn
[Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein]
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Other_names
[proliferating cell nuclear antigen; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; proliferating cell nuclear antigen; Cyclin]
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Products_gene_name
[PCNA]
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Other_gene_names
[PCNA; PCNA; ATLD2; PCNA]
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Clonality
Monoclonal
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Clone
[SPM350]
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Reactivity
Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, Yeast (S. pombe and S. cerevisiae)
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Specificity
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
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Form
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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Storage_stability
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
PCNA, PCNA-AS1
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Short name
PCNA (Proliferating Antigen) (G1- & S-phase ) Mouse Monoclonal Antibody
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Technique
Mouse monoclonal, Antibody, antigen, Mouse, antibodies against human proteins, antibodies for, antigenes, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA. Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
Mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG2a, kappa
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Species
Mouse, Mouses
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Alternative name
PCNA (Proliferating cellular Nuclear protein) (G1- & S-phase detection labelled) Mouse monoclonal (antibody to-)
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Alternative technique
antibodies, antigenes, murine
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Tissue
cell
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