NGF-beta, human recombinant

  • Catalog number
    4303R-1000
  • Price
    Please ask
  • Size
    1 mg
  • Synonyms
    β Polypeptide, NGF, NGFB, HSAN5, β-NGF, MGC161426, MGC161428.
  • Alternative_names
    β Polypeptide, NGF, NGFB, HSAN5, β-NGF, MGC161426, MGC161428.
  • Description
    A factor critical for the survival & maintenance of sympathetic and sensory neurons
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥97%
  • Assay
    SDS-PAGE
  • Purity
    ≥97%
  • Biological activity
    The ED₅₀ as determined by the ability to stimulate neurite growth in chick E9 DRG cells is
  • Results
    0.8 ng/ml
  • Molecular Weight
    13.6 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized protein
  • Physical form description
    Sterile filtered and then lyophilized with no additives.
  • Reconstitution Instructions
    Reconstituted human NGF-β should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.
  • Background Information
    Nerve growth factor-β (NGF-β) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-β is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular weight of 16.95 kDa. The protein is purified by proprietary chromatographic techniques.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    NGF, NGF-AS1
  • Short name
    NGF-beta, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    nerve growth factor (beta polypeptide)-b, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    nerve growth factor (beta polypeptide), Beta-NGF and HSAN5 and NGFB, NGF and IDBG-101347 and ENSG00000134259 and 4803, growth factor activity, Extracellular, Ngf and IDBG-180362 and ENSMUSG00000027859 and 18049, NGF and IDBG-648895 and ENSBTAG00000007446 and 281350
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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