NGF-beta, human recombinant
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Catalog number
4303R-1000
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Price
Please ask
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Size
1 mg
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Synonyms
β Polypeptide, NGF, NGFB, HSAN5, β-NGF, MGC161426, MGC161428.
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Alternative_names
β Polypeptide, NGF, NGFB, HSAN5, β-NGF, MGC161426, MGC161428.
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Description
A factor critical for the survival & maintenance of sympathetic and sensory neurons
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥97%
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Assay
SDS-PAGE
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Purity
≥97%
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Biological activity
The ED₅₀ as determined by the ability to stimulate neurite growth in chick E9 DRG cells is
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Results
0.8 ng/ml
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Molecular Weight
13.6 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Sterile filtered and then lyophilized with no additives.
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Reconstitution Instructions
Reconstituted human NGF-β should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.
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Background Information
Nerve growth factor-β (NGF-β) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-β is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular weight of 16.95 kDa. The protein is purified by proprietary chromatographic techniques.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NGF, NGF-AS1
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Short name
NGF-beta, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
nerve growth factor (beta polypeptide)-b, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
nerve growth factor (beta polypeptide), Beta-NGF and HSAN5 and NGFB, NGF and IDBG-101347 and ENSG00000134259 and 4803, growth factor activity, Extracellular, Ngf and IDBG-180362 and ENSMUSG00000027859 and 18049, NGF and IDBG-648895 and ENSBTAG00000007446 and 281350
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Gene info
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Identity
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Gene
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Long gene name
nerve growth factor
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Synonyms gene
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Synonyms gene name
- nerve growth factor (beta polypeptide)
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
NGF antisense RNA 1
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Synonyms
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Locus
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Discovery year
2018-06-08
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Pubmed identfication
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Classification
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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