Anti-Cytochrome C (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-0013R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideCytochrome C
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SpecificityThis antibody reacts specifically with Cytochrome C
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsMitochondrion
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Antigen SourceKLH conjugated synthetic peptide derived from human Cytochrome C
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Gene ID54205
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Swiss ProtP99999
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ApplicationsIF(IHC-P), IF(ICC)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsCYC; HCS; THC4; Cytochrome c; CYCS
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Also known asCytochrome C Polyclonal Antibody
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Other nameAnti-Cytochrome C Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolCOX11, COX5A, COX17
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Short nameAnti-Cytochrome C
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameCytochrome C Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene namecytochrome c oxidase copper chaperone COX11
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Synonyms gene name
- COX11 (yeast) homolog, cytochrome c oxidase assembly protein
- cytochrome c oxidase assembly homolog 11 (yeast)
- COX11, cytochrome c oxidase copper chaperone
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial respiratory chain complex assembly factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome c oxidase subunit 5A
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Synonyms gene name
- cytochrome c oxidase subunit Va
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GenBank acession
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Locus
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Discovery year1998-12-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial complex IV: cytochrome c oxidase subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome c oxidase copper chaperone COX17
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Synonyms gene name
- COX17 (yeast) homolog, cytochrome c oxidase assembly protein
- COX17 cytochrome c oxidase assembly homolog (S. cerevisiae)
- COX17, cytochrome c oxidase copper chaperone
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial respiratory chain complex assembly factors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data