CPLA2 Antibody
-
Catalog numberR31157
-
PricePlease ask
-
Size0.1mg
-
-
CategoryAntibody
-
Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
-
FormAntigen affinity purified
-
ConjugationUnconjugated
-
ClonePolyclonal antibody
-
Recognised antigenCPLA2
-
Host animalRabbit (Oryctolagus cuniculus)
-
ClonalityPolyclonal (rabbit origin)
-
Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applicationsWB
-
Recommended dilutionsWestern blot: 0.5-1ug/ml
-
NotesThe stated application concentrations are suggested starting amounts. Titration of the CPLA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented useThis CPLA2 antibodyis to be used only for research purposes and not for diagnostics..
-
UniprotP47712
-
PurityAntigen affinity
-
DescriptionCytosolic Phospholipase A2, Group IVA is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al.(1995) mapped the gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al.(1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands(Skorecki, 1995).
-
ImmunogenAn amino acid sequence from the C-terminus of human Phospholipase A2 (PNQAFKRLHDLMHFN) was used as the immunogen for this CPLA2 antibody.
-
StorageAfter reconstitution, the CPLA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
-
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolPLA2G4C, PLA2G4A, PLA2G4B, KAT5
-
Short nameAnti-CPLA2
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative nameAntibodies to CPLA2
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namephospholipase A2 group IVC
-
Synonyms gene name
- phospholipase A2, group IVC (cytosolic, calcium-independent)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-11-20
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Phospholipases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namephospholipase A2 group IVA
-
Synonyms gene
-
Synonyms gene name
- phospholipase A2, group IVA (cytosolic, calcium-dependent)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1994-11-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Phospholipases
- C2 domain containing phospholipases
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namephospholipase A2 group IVB
-
Synonyms gene name
- phospholipase A2, group IVB (cytosolic)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-11-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- C2 domain containing phospholipases
- Phospholipases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namelysine acetyltransferase 5
-
Synonyms gene
-
Synonyms gene name
- HIV-1 Tat interactive protein, 60kDa
- K(lysine) acetyltransferase 5
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2000-04-13
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Zinc fingers C2HC-type
- MYST type domain containing lysine acetyltransferases
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data