anti-sRANK Ligand Antibody
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Catalog number
XP-5273Bt
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Price
Please ask
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Size
0.05 mg
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Gene names
ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF
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Antibodie s label
Biotin
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Purity
Anti-hsRANKL specific antibody was purified by affinity chromatography and then biotinylated.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsRANKL (Human sRANK-Ligand).
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Immuno application
ELISA, Western Blot
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Antibody advice
ELISA:To detect hsRANKL by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hsRANKL.Sandwich:To detect hsRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sRANKL (XP-5273) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL. Western Blot:To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Keep at
sRANK Ligand antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works with
Human
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Omim nr
12643360
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Ncbi nr
O14788
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Vial with antibody
Lyophilized
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NCBI GENE nr
8600
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GENE symbol
TNFSF11
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Antigene name
tumor necrosis factor (ligand) superfamily, member 11
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Source
Homo sapiens
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Protein nr
O14788
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
anti-sRANK Ligand Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody tosRANK Ligand (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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