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Available ordering format
Liquid in PBS pH 8.0, 0.02% sodium azide
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Immunogen
KLH-conjugated synthetic peptide derived from available di and monocot PsbS sequences, including Arabidopsis thaliana (At1g44575). This sequence is even conserved in conifers.
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS09 533 | anti-PsbS rabbit antibodyPSII available antibodies against Photosystem II proteins
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Recommended dilutions for use
1: 2000 - 1 : 4000 (WB)
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Molecular weight expected аpparent
28 | 22 kDa for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana, Avena sativa, Brassica napus, Hordeum vulgare, Oryza sativa, Pinus sylvestris, Populus tremula, Spinacia oleracea
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Possible reactivity
dicots and monocots, conifers
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No reactivity
Chlamydomonas reinhardtii, Chlorella sp.
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Supplementary information
to be added when available
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References
Hubbart et al. (2012). The photoprotective protein PsbS exerts control over CO2 assimilation rate in fluctuating light in rice. The Plant J. March 2012.
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Scientific context
The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence.
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Notes
None
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Protein number
Q9XF91
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TAIR number
AT1G44575
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Description
The kilo Daltons subunit weight of PsbS, 22 Lhc-like PSII protein compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein